{"id":317,"date":"2020-03-02T13:12:42","date_gmt":"2020-03-02T12:12:42","guid":{"rendered":"https:\/\/zencellowl.com\/?page_id=317"},"modified":"2026-05-28T16:58:27","modified_gmt":"2026-05-28T14:58:27","slug":"cytotoxicity-assay","status":"publish","type":"page","link":"https:\/\/zencellowl.com\/de\/cytotoxicity-assay\/","title":{"rendered":"Zytotoxizit\u00e4tsanalyse"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"317\" class=\"elementor elementor-317\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-5882d69 e-flex e-con-boxed e-con e-parent\" data-id=\"5882d69\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-b3fa041 elementor-widget elementor-widget-html\" data-id=\"b3fa041\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"html.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<link href=\"https:\/\/fonts.googleapis.com\/css2?family=Montserrat:wght@300;400;500;600;700;800&display=swap\" rel=\"stylesheet\">\n\n<style>\n.ica-full *, .ica-full *::before, .ica-full *::after { \n    box-sizing: border-box; \n    font-family: 'Montserrat', sans-serif !important; \n}\n.ica-full { color: #2c3e50; }\n.ica-full .ica-kicker { font-size: 11px; font-weight: 700; letter-spacing: .1em; text-transform: uppercase; color: #3aaea0; margin-bottom: 10px; }\n.ica-full h2 { font-size: clamp(1.8rem, 3vw, 2.6rem) !important; font-weight: 800 !important; color: #1a2e3a !important; margin-bottom: 1rem !important; line-height: 1.18 !important; letter-spacing: -.3px; }\n.ica-full h2 span { color: #3aaea0; }\n.ica-intro { font-size: 17px; color: #5a6a7a; max-width: 650px; line-height: 1.75; margin-bottom: 3rem; }\n.ica-sec-full { max-width: 1140px; margin: 0 auto; padding: 5rem 2rem; }\n\n\/* HERO *\/\n.ica-hero-full { background: linear-gradient(135deg, #ffffff 0%, #f0f9f8 100%); padding: 5rem 2rem 4rem; text-align: center; position: relative; overflow: hidden; }\n.ica-hero-full::after { content: ''; position: absolute; inset: 0; background-image: radial-gradient(circle, rgba(58,174,160,0.03) 1px, transparent 1px); background-size: 32px 32px; pointer-events: none; }\n.ica-hero-full-in { position: relative; z-index: 1; max-width: 780px; margin: 0 auto; }\n.ica-hero-full .ica-pill { display: inline-flex; align-items: center; gap: 7px; background: rgba(58,174,160,0.1); border: 1.5px solid #3aaea0; color: #2d8f83; border-radius: 100px; padding: 8px 20px; font-size: 11px; font-weight: 700; letter-spacing: .08em; text-transform: uppercase; margin-bottom: 1.5rem; }\n.ica-hero-full h1 { font-size: clamp(2.2rem, 5vw, 3.8rem) !important; font-weight: 800 !important; line-height: 1.15 !important; color: #1a2e3a !important; margin-bottom: 1.5rem !important; letter-spacing: -0.5px; }\n.ica-hero-full h1 span { color: #3aaea0; }\n.ica-hero-full-sub { font-size: 19px !important; color: #5a6a7a !important; line-height: 1.7; max-width: 650px; margin: 0 auto 2.5rem !important; }\n.ica-hero-full-btns { display: flex; gap: 14px; justify-content: center; flex-wrap: wrap; margin-bottom: 3.5rem; }\n.ica-hero-full .ica-btn-main { background: #3aaea0; color: white !important; padding: 16px 32px; border-radius: 10px; font-size: 16px; font-weight: 700; text-decoration: none !important; transition: all .3s ease; display: inline-flex; align-items: center; gap: 8px; box-shadow: 0 4px 16px rgba(58,174,160,0.25); }\n.ica-hero-full .ica-btn-main:hover { background: #2d8f83; transform: translateY(-2px); box-shadow: 0 6px 20px rgba(58,174,160,0.35); }\n.ica-hero-full .ica-btn-ghost { background: #ffffff; color: #3aaea0 !important; padding: 16px 32px; border-radius: 10px; font-size: 16px; font-weight: 700; text-decoration: none !important; border: 2px solid #3aaea0; transition: all .3s ease; display: inline-flex; align-items: center; gap: 8px; }\n.ica-hero-full .ica-btn-ghost:hover { background: #f0f9f8; transform: translateY(-2px); }\n.ica-hero-full-stats { display: grid; grid-template-columns: repeat(4, 1fr); gap: 20px; max-width: 900px; margin: 0 auto; }\n.ica-hero-full .ica-hstat { background: #ffffff; padding: 1.5rem 1rem; border-radius: 12px; border: 1.5px solid #e8ecee; transition: all .3s ease; }\n.ica-hero-full .ica-hstat:hover { border-color: #3aaea0; box-shadow: 0 4px 16px rgba(58,174,160,0.12); transform: translateY(-2px); }\n.ica-hero-full .ica-hstat-n { font-size: 2.6rem; font-weight: 800; color: #3aaea0; line-height: 1; margin-bottom: 8px; }\n.ica-hero-full .ica-hstat-l { font-size: 12px; color: #7f8c8d; line-height: 1.4; font-weight: 500; }\n\n\/* PAIN *\/\n.ica-pain-full { background: #ffffff; }\n.ica-pain-full-grid { display: grid; grid-template-columns: repeat(3, 1fr); gap: 20px; }\n.ica-pain-full-card { background: #ffffff; border: 2px solid #fee; border-radius: 16px; padding: 2rem; border-left: 5px solid #e74c3c; transition: all .3s ease; box-shadow: 0 2px 8px rgba(231,76,60,0.08); }\n.ica-pain-full-card:hover { border-color: #e74c3c; box-shadow: 0 8px 24px rgba(231,76,60,0.15); transform: translateY(-4px); }\n.ica-pain-full-card .icon { font-size: 36px; margin-bottom: 16px; display: block; }\n.ica-pain-full-card .title { font-size: 16px; font-weight: 700; color: #1a2e3a; margin-bottom: 10px; line-height: 1.3; }\n.ica-pain-full-card .desc { font-size: 14px; color: #5a6a7a; line-height: 1.7; margin: 0; }\n\n\/* VS *\/\n.ica-vs-full { background: linear-gradient(135deg, #f0f9f8 0%, #ffffff 100%); }\n.ica-vs-full-wrap { display: grid; grid-template-columns: 1fr 60px 1fr; gap: 0; align-items: stretch; margin-bottom: 2.5rem; }\n.ica-vs-full-card { border-radius: 20px; overflow: hidden; border: 2px solid #e8ecee; background: #ffffff; box-shadow: 0 4px 16px rgba(0,0,0,0.04); }\n.ica-vs-full-card.win { border-color: #3aaea0; box-shadow: 0 12px 40px rgba(58,174,160,0.2); transform: scale(1.02); }\n.ica-vs-full-head { padding: 20px 28px; font-size: 15px; font-weight: 700; }\n.ica-vs-full-card.win .ica-vs-full-head { background: linear-gradient(135deg, #3aaea0 0%, #2d8f83 100%); color: white; }\n.ica-vs-full-card:not(.win) .ica-vs-full-head { background: #f8fafa; color: #5a6a7a; }\n.ica-vs-full-row { display: flex; justify-content: space-between; align-items: center; padding: 14px 28px; border-top: 1px solid #f0f0f0; font-size: 13.5px; transition: background .2s ease; }\n.ica-vs-full-row:hover { background: #fafbfb; }\n.ica-vs-full-row:nth-child(even) { background: #f8f9fa; }\n.ica-vs-full-row:nth-child(even):hover { background: #f0f2f4; }\n.ica-vs-full-row .lbl { color: #5a6a7a; font-weight: 500; }\n.ica-vs-full-row .val { font-weight: 700; color: #1a2e3a; }\n.ica-vs-full-row .val.g { color: #27ae60; }\n.ica-vs-full-row .val.r { color: #e74c3c; }\n.ica-vs-full-mid { display: flex; align-items: center; justify-content: center; }\n.ica-vs-full-vs { width: 60px; height: 60px; background: linear-gradient(135deg, #3aaea0 0%, #2d8f83 100%); border-radius: 50%; color: white; font-size: 14px; font-weight: 800; display: flex; align-items: center; justify-content: center; box-shadow: 0 6px 20px rgba(58,174,160,0.3); }\n.ica-vs-full-savings { background: linear-gradient(135deg, #27ae60 0%, #229954 100%); color: white; border-radius: 20px; padding: 2.5rem 3rem; text-align: center; margin-top: 2rem; box-shadow: 0 10px 30px rgba(39,174,96,0.25); }\n.ica-vs-full-savings-n { font-size: 4.2rem; font-weight: 800; letter-spacing: -2px; line-height: 1; margin-bottom: 12px; }\n.ica-vs-full-savings-l { font-size: 16px; opacity: .95; line-height: 1.6; }\n\n\/* FEATURES *\/\n.ica-feat-full { background: #ffffff; }\n.ica-feat-full-grid { display: grid; grid-template-columns: repeat(3, 1fr); gap: 20px; }\n.ica-feat-full-card { background: #ffffff; border: 2px solid #e8ecee; border-radius: 16px; padding: 2rem; transition: all .3s ease; box-shadow: 0 2px 8px rgba(58,174,160,0.04); }\n.ica-feat-full-card:hover { border-color: #3aaea0; box-shadow: 0 8px 24px rgba(58,174,160,0.12); transform: translateY(-4px); }\n.ica-feat-full-card.star { border-color: #3aaea0; background: linear-gradient(135deg, #f0f9f8 0%, #ffffff 100%); }\n.ica-feat-full-card .icon { font-size: 36px; margin-bottom: 16px; display: block; }\n.ica-feat-full-badge { display: inline-block; font-size: 10px; font-weight: 700; background: #3aaea0; color: white; padding: 3px 10px; border-radius: 4px; margin-left: 8px; vertical-align: middle; letter-spacing: 0.05em; }\n.ica-feat-full-card .title { font-size: 16px; font-weight: 700; color: #1a2e3a; margin-bottom: 10px; line-height: 1.3; }\n.ica-feat-full-card .desc { font-size: 14px; color: #5a6a7a; line-height: 1.7; margin: 0; }\n\n\/* AUTOFOCUS \/ VESSELS \u2014 new section, same style *\/\n.ica-setup-full { background: linear-gradient(135deg, #f0f9f8 0%, #ffffff 100%); }\n.ica-setup-grid { display: grid; grid-template-columns: repeat(3, 1fr); gap: 20px; margin-bottom: 2.5rem; }\n.ica-setup-card { background: #ffffff; border: 2px solid #e8ecee; border-radius: 16px; padding: 2rem; transition: all .3s ease; box-shadow: 0 2px 8px rgba(58,174,160,0.04); }\n.ica-setup-card:hover { border-color: #3aaea0; box-shadow: 0 8px 24px rgba(58,174,160,0.12); transform: translateY(-4px); }\n.ica-setup-card.star { border-color: #3aaea0; background: linear-gradient(135deg, #f0f9f8 0%, #ffffff 100%); }\n.ica-setup-card .icon { font-size: 36px; margin-bottom: 16px; display: block; }\n.ica-setup-card .title { font-size: 16px; font-weight: 700; color: #1a2e3a; margin-bottom: 10px; line-height: 1.3; }\n.ica-setup-card .desc { font-size: 14px; color: #5a6a7a; line-height: 1.7; margin: 0 0 12px; }\n.ica-setup-card .tag { display: inline-block; font-size: 11px; font-weight: 700; background: rgba(58,174,160,0.1); color: #2d8f83; padding: 4px 12px; border-radius: 100px; border: 1px solid rgba(58,174,160,0.25); }\n.ica-vessel-table { width: 100%; border-collapse: separate; border-spacing: 0; background: #ffffff; border-radius: 16px; overflow: hidden; box-shadow: 0 4px 16px rgba(0,0,0,0.04); border: 2px solid #e8ecee; }\n.ica-vessel-table th { background: #1a2e3a; color: #fff; padding: 14px 20px; text-align: left; font-size: 12px; font-weight: 700; letter-spacing: .05em; text-transform: uppercase; }\n.ica-vessel-table th.hl { background: #3aaea0; }\n.ica-vessel-table td { padding: 13px 20px; font-size: 13.5px; border-top: 1px solid #f0f0f0; }\n.ica-vessel-table td:first-child { font-weight: 700; color: #1a2e3a; background: #f8fafa; }\n.ica-vessel-table td.g { color: #27ae60; font-weight: 700; }\n.ica-vessel-table td.m { color: #3aaea0; font-weight: 500; }\n.ica-vessel-table td.dim { color: #95a5a6; }\n\n\/* SCAN SPEED \u2014 new section, same style *\/\n.ica-scan-full { background: #ffffff; }\n.ica-scan-grid { display: grid; grid-template-columns: repeat(3, 1fr); gap: 20px; margin-bottom: 2rem; }\n.ica-scan-card { border-radius: 16px; padding: 2rem; text-align: center; }\n.ica-scan-card.dark { background: linear-gradient(135deg, #3aaea0 0%, #2d8f83 100%); color: white; box-shadow: 0 8px 24px rgba(58,174,160,0.25); }\n.ica-scan-card.border { background: #ffffff; border: 2px solid #3aaea0; box-shadow: 0 4px 16px rgba(58,174,160,0.12); }\n.ica-scan-card.light { background: #f8fafa; border: 2px solid #e8ecee; }\n.ica-scan-label { font-size: 11px; font-weight: 700; letter-spacing: .1em; text-transform: uppercase; margin-bottom: 10px; opacity: .75; }\n.ica-scan-card.dark .ica-scan-label { color: rgba(255,255,255,.8); }\n.ica-scan-card:not(.dark) .ica-scan-label { color: #3aaea0; }\n.ica-scan-num { font-size: 3rem; font-weight: 800; line-height: 1; margin-bottom: 6px; letter-spacing: -1px; }\n.ica-scan-card.dark .ica-scan-num { color: #ffffff; }\n.ica-scan-card:not(.dark) .ica-scan-num { color: #3aaea0; }\n.ica-scan-sub { font-size: 14px; font-weight: 600; margin-bottom: 10px; }\n.ica-scan-card.dark .ica-scan-sub { color: rgba(255,255,255,.9); }\n.ica-scan-card:not(.dark) .ica-scan-sub { color: #1a2e3a; }\n.ica-scan-desc { font-size: 13px; line-height: 1.6; }\n.ica-scan-card.dark .ica-scan-desc { color: rgba(255,255,255,.75); }\n.ica-scan-card:not(.dark) .ica-scan-desc { color: #7f8c8d; }\n.ica-scan-note { background: #f0f9f8; border-left: 4px solid #3aaea0; border-radius: 0 12px 12px 0; padding: 1.25rem 1.5rem; display: flex; align-items: center; gap: 14px; flex-wrap: wrap; }\n.ica-scan-note p { font-size: 14px; color: #5a6a7a; margin: 0; line-height: 1.6; flex: 1; }\n.ica-scan-note a { display: inline-block; background: #3aaea0; color: white !important; padding: 10px 20px; border-radius: 8px; font-size: 13px; font-weight: 700; text-decoration: none !important; white-space: nowrap; flex-shrink: 0; transition: background .2s; }\n.ica-scan-note a:hover { background: #2d8f83; }\n\n\/* CALCULATOR *\/\n.ica-calc-full { background: linear-gradient(135deg, #f0f9f8 0%, #e8f5f4 100%); position: relative; }\n.ica-calc-full::before { content: ''; position: absolute; inset: 0; background-image: radial-gradient(circle, rgba(58,174,160,0.04) 1px, transparent 1px); background-size: 32px 32px; pointer-events: none; }\n.ica-calc-full-in { max-width: 1000px; margin: 0 auto; position: relative; z-index: 1; }\n.ica-calc-full .ica-kicker, .ica-calc-full h2 { text-align: center; }\n.ica-calc-full-subtitle { font-size: 17px; color: #5a6a7a; text-align: center; max-width: 600px; margin: 0 auto 3rem; line-height: 1.6; }\n.ica-calc-box-full { background: #ffffff; border-radius: 20px; padding: 3rem; box-shadow: 0 10px 40px rgba(58,174,160,0.12); border: 2px solid #e8f5f4; }\n.ica-calc-full-grid { display: grid; grid-template-columns: 1fr 1fr; gap: 3rem; align-items: start; }\n.ica-calc-full-left h3, .ica-calc-full-right h3 { font-size: 18px; font-weight: 700; color: #1a2e3a; margin-bottom: 1.5rem; }\n.ica-slider-full-wrap { margin-bottom: 2rem; background: #f8fafa; padding: 1.25rem; border-radius: 12px; border: 1px solid #e8ecee; }\n.ica-slider-full-label { display: flex; justify-content: space-between; align-items: center; margin-bottom: 12px; }\n.ica-slider-full-name { font-size: 14px; color: #2c3e50; font-weight: 600; }\n.ica-slider-full-val { font-size: 22px; font-weight: 800; color: #3aaea0; }\ninput[type=range].ica-range-full { width: 100%; height: 6px; background: #e8ecee; border-radius: 3px; outline: none; -webkit-appearance: none; appearance: none; cursor: pointer; }\ninput[type=range].ica-range-full::-webkit-slider-thumb { -webkit-appearance: none; appearance: none; width: 24px; height: 24px; border-radius: 50%; background: #3aaea0; cursor: pointer; box-shadow: 0 2px 8px rgba(58,174,160,0.3); border: 3px solid #ffffff; }\ninput[type=range].ica-range-full::-moz-range-thumb { width: 24px; height: 24px; border-radius: 50%; background: #3aaea0; cursor: pointer; box-shadow: 0 2px 8px rgba(58,174,160,0.3); border: 3px solid #ffffff; }\n.ica-result-full-card { background: linear-gradient(135deg, #f8fafa 0%, #ffffff 100%); border-radius: 12px; padding: 1.5rem; margin-bottom: 1rem; border: 1.5px solid #e8ecee; transition: all 0.2s; }\n.ica-result-full-card:hover { border-color: #3aaea0; box-shadow: 0 4px 16px rgba(58,174,160,0.1); }\n.ica-result-full-label { font-size: 12px; color: #7f8c8d; margin-bottom: 8px; font-weight: 600; text-transform: uppercase; letter-spacing: .05em; }\n.ica-result-full-val { font-size: 28px; font-weight: 800; color: #1a2e3a; line-height: 1; margin-bottom: 6px; }\n.ica-result-full-val.red { color: #e74c3c; }\n.ica-result-full-val.green { color: #27ae60; }\n.ica-result-full-sub { font-size: 13px; color: #95a5a6; margin-top: 4px; }\n.ica-savings-full { background: linear-gradient(135deg, #3aaea0 0%, #2d8f83 100%); border-radius: 16px; padding: 2rem; text-align: center; margin-top: 1rem; box-shadow: 0 8px 24px rgba(58,174,160,0.25); }\n.ica-savings-full-label { font-size: 13px; color: rgba(255,255,255,0.8); font-weight: 700; text-transform: uppercase; letter-spacing: 0.1em; margin-bottom: 8px; }\n.ica-savings-full-val { font-size: 48px; font-weight: 800; color: #ffffff; line-height: 1; margin-bottom: 12px; letter-spacing: -1px; }\n.ica-savings-full-desc { font-size: 15px; color: rgba(255,255,255,0.9); line-height: 1.5; }\n.ica-calc-full-note { font-size: 13px; color: #95a5a6; line-height: 1.6; margin-top: 2rem; padding: 1rem; background: #f8fafa; border-radius: 8px; border-left: 3px solid #3aaea0; }\n\n\/* TESTIMONIALS *\/\n.ica-testi-full { background: #ffffff; }\n.ica-testi-full-grid { display: grid; grid-template-columns: repeat(3, 1fr); gap: 20px; }\n.ica-testi-full-card { background: #ffffff; border: 2px solid #e8ecee; border-radius: 16px; padding: 2rem; transition: all .3s ease; box-shadow: 0 2px 8px rgba(58,174,160,0.04); }\n.ica-testi-full-card:hover { border-color: #3aaea0; box-shadow: 0 8px 24px rgba(58,174,160,0.12); transform: translateY(-4px); }\n.ica-testi-full-stars { color: #f5a623; font-size: 16px; margin-bottom: 12px; letter-spacing: 2px; }\n.ica-testi-full-text { font-size: 14px; color: #5a6a7a; line-height: 1.7; margin-bottom: 1.25rem; font-style: italic; }\n.ica-testi-full-name { font-size: 14px; font-weight: 700; color: #1a2e3a; margin-bottom: 4px; }\n.ica-testi-full-role { font-size: 13px; color: #95a5a6; }\n\n\/* PUBLICATIONS *\/\n.ica-pub-full { background: linear-gradient(135deg, #f0f9f8 0%, #e8f5f4 100%); border-top: 2px solid #e8ecee; border-bottom: 2px solid #e8ecee; padding: 4rem 2rem; text-align: center; }\n.ica-pub-full-num { font-size: 4rem; font-weight: 800; color: #3aaea0; line-height: 1; margin-bottom: 12px; }\n.ica-pub-full-label { font-size: 16px; color: #5a6a7a; max-width: 600px; margin: 0 auto 1.5rem; line-height: 1.6; }\n.ica-pub-full-link { color: #3aaea0; font-size: 15px; font-weight: 700; text-decoration: none; transition: all 0.2s; }\n.ica-pub-full-link:hover { color: #2d8f83; }\n\n\/* FAQ *\/\n.ica-faq-full { background: #ffffff; }\n.ica-faq-full-list { max-width: 800px; }\n.ica-faq-full-item { border-bottom: 2px solid #e8ecee; transition: border-color 0.2s; }\n.ica-faq-full-item:hover { border-color: #3aaea0; }\n.ica-faq-full-q { padding: 20px 0; font-size: 16px; font-weight: 600; color: #1a2e3a; cursor: pointer; display: flex; justify-content: space-between; align-items: center; gap: 1.5rem; transition: color 0.2s; }\n.ica-faq-full-q:hover { color: #3aaea0; }\n.ica-faq-full-ico { font-size: 24px; font-weight: 300; color: #3aaea0; transition: transform 0.3s; flex-shrink: 0; }\n.ica-faq-full-item.open .ica-faq-full-ico { transform: rotate(45deg); }\n.ica-faq-full-a { font-size: 14px; color: #5a6a7a; line-height: 1.7; max-height: 0; overflow: hidden; transition: max-height 0.4s ease, padding-bottom 0.4s; }\n.ica-faq-full-item.open .ica-faq-full-a { max-height: 600px; padding-bottom: 20px; }\n\n\/* CTA *\/\n.ica-cta-full { background: linear-gradient(135deg, #3aaea0 0%, #2d8f83 100%); padding: 5rem 2rem; text-align: center; color: white; position: relative; }\n.ica-cta-full::before { content: ''; position: absolute; inset: 0; background-image: radial-gradient(circle, rgba(255,255,255,0.05) 1px, transparent 1px); background-size: 32px 32px; pointer-events: none; }\n.ica-cta-full-in { max-width: 800px; margin: 0 auto; position: relative; z-index: 1; }\n.ica-cta-full h2 { color: white !important; margin-bottom: 1.5rem !important; }\n.ica-cta-full-text { font-size: 18px; color: rgba(255,255,255,0.9); margin-bottom: 2.5rem; line-height: 1.7; }\n.ica-cta-full-btns { display: flex; gap: 14px; justify-content: center; flex-wrap: wrap; margin-bottom: 2.5rem; }\n.ica-cta-full .ica-btn-white { background: #ffffff; color: #3aaea0 !important; padding: 18px 36px; border-radius: 10px; font-size: 16px; font-weight: 700; text-decoration: none !important; transition: all 0.3s; box-shadow: 0 4px 16px rgba(0,0,0,0.1); }\n.ica-cta-full .ica-btn-white:hover { background: #f0f9f8; transform: translateY(-2px); box-shadow: 0 6px 20px rgba(0,0,0,0.15); }\n.ica-cta-full .ica-btn-outline { background: transparent; color: white !important; padding: 18px 36px; border-radius: 10px; font-size: 16px; font-weight: 700; text-decoration: none !important; border: 2px solid white; transition: all 0.3s; }\n.ica-cta-full .ica-btn-outline:hover { background: rgba(255,255,255,0.1); transform: translateY(-2px); }\n.ica-cta-full-trust { display: flex; gap: 2.5rem; justify-content: center; flex-wrap: wrap; font-size: 14px; opacity: 0.9; font-weight: 500; }\n.ica-cta-full-trust span::before { content: \"\u2713 \"; font-weight: 700; }\n\n\/* RESPONSIVE *\/\n@media (max-width: 900px) {\n    .ica-hero-full-stats { grid-template-columns: repeat(2, 1fr); }\n    .ica-pain-full-grid, .ica-feat-full-grid, .ica-testi-full-grid, .ica-setup-grid, .ica-scan-grid { grid-template-columns: 1fr 1fr; }\n    .ica-vs-full-wrap { grid-template-columns: 1fr; gap: 20px; }\n    .ica-vs-full-mid { display: none; }\n    .ica-vs-full-card.win { transform: none; }\n    .ica-calc-full-grid { grid-template-columns: 1fr; gap: 2rem; }\n    .ica-calc-box-full { padding: 2rem; }\n}\n@media (max-width: 600px) {\n    .ica-sec-full { padding: 3rem 1.5rem; }\n    .ica-hero-full-stats { grid-template-columns: 1fr; gap: 12px; }\n    .ica-hero-full h1 { font-size: 2rem !important; }\n    .ica-pain-full-grid, .ica-feat-full-grid, .ica-testi-full-grid, .ica-setup-grid, .ica-scan-grid { grid-template-columns: 1fr; }\n    .ica-vs-full-savings { padding: 2rem; }\n    .ica-vs-full-savings-n { font-size: 3rem; }\n    .ica-calc-box-full { padding: 1.5rem; }\n    .ica-savings-full-val { font-size: 36px; }\n    .ica-cta-full-btns { flex-direction: column; align-items: stretch; }\n}\n<\/style><style>\n\/* \u2500\u2500 APP PAGE EXTRAS \u2500\u2500 *\/\n.app-img { width:100%; border-radius:16px; display:block; box-shadow:0 6px 28px rgba(15,47,58,.10); }\n.app-img-wrap { border-radius:16px; overflow:hidden; box-shadow:0 6px 28px rgba(15,47,58,.10); }\n.app-feature-row { display:grid; grid-template-columns:1fr 1fr; gap:52px; align-items:center; margin-bottom:64px; }\n.app-feature-row:last-child { margin-bottom:0; }\n.app-feature-text h3 { font-size:1.4rem !important; font-weight:800 !important; color:#1a2e3a !important; margin-bottom:14px; font-family:'Montserrat',sans-serif; }\n.app-feature-text p { font-size:14px; color:#5a6a7a; line-height:1.75; margin-bottom:14px; font-family:'Montserrat',sans-serif; }\n.app-feature-text ul { list-style:none; padding:0; margin:0; }\n.app-feature-text ul li { font-size:13.5px; color:#5a6a7a; padding:5px 0 5px 22px; position:relative; font-family:'Montserrat',sans-serif; }\n.app-feature-text ul li::before { content:'\u2713'; position:absolute; left:0; color:#3aaea0; font-weight:700; }\n.app-img-caption { font-size:11.5px; color:#95a5a6; margin-top:8px; text-align:center; font-family:'Montserrat',sans-serif; font-style:italic; line-height:1.5; }\n.app-example-grid { display:grid; grid-template-columns:1fr 1fr; gap:20px; margin-top:2rem; }\n.app-example-card { background:#fff; border:1px solid #e8ecee; border-radius:14px; overflow:hidden; box-shadow:0 2px 8px rgba(58,174,160,.04); }\n.app-example-card img { width:100%; display:block; }\n.app-example-body { padding:14px 16px; }\n.app-example-body .tag { font-size:10px; font-weight:700; letter-spacing:.1em; text-transform:uppercase; color:#3aaea0; margin-bottom:6px; display:block; font-family:'Montserrat',sans-serif; }\n.app-example-body p { font-size:12.5px; color:#5a6a7a; line-height:1.6; margin:0; font-family:'Montserrat',sans-serif; }\n.app-adv-grid { display:grid; grid-template-columns:repeat(3,1fr); gap:20px; }\n@media(max-width:900px) {\n  .app-feature-row { grid-template-columns:1fr; gap:28px; }\n  .app-adv-grid, .app-example-grid { grid-template-columns:1fr 1fr; }\n}\n@media(max-width:600px) {\n  .app-adv-grid { grid-template-columns:1fr; }\n  .app-example-grid { grid-template-columns:1fr; }\n}\n<\/style>\n\n<div class=\"ica-full\">\n\n<!-- \u2550\u2550\u2550 SEO META COMMENT \u2550\u2550\u2550\n  URL: zencellowl.com\/cytotoxicity-assay\/\n  Yoast Title: Cytotoxicity Assay with Live Cell Imaging | zenCELL owl\n  Yoast Desc: Monitor drug effects on up to 24 cell cultures simultaneously \u2014 24\/7, label-free, non-invasive. Kinetic cytotoxicity data with zenCELL owl. Free demo available.\n  Focus Keyphrase: cytotoxicity assay live cell imaging\n-->\n\n<!-- \u2550\u2550\u2550 1. HERO \u2550\u2550\u2550 -->\n<div class=\"ica-hero-full\">\n  <div class=\"ica-hero-full-in\">\n    <div class=\"ica-pill\">\ud83d\udc8a Application \u2014 Cytotoxicity Assay<\/div>\n    <h1>Cytotoxicity Assay \u2014<br><span>Kinetic, Label-Free, 24 Wells in Parallel<\/span><\/h1>\n    <p class=\"ica-hero-full-sub\">\n      Monitor morphological drug effects on up to 24 cell cultures simultaneously \u2014 24\/7 inside the incubator. No endpoint assay. No cell removal. Continuous kinetic data from first treatment to cell death.\n    <\/p>\n    <div class=\"ica-hero-full-btns\">\n      <a href=\"https:\/\/zencellowl.com\/live-remotedemo\/\" class=\"ica-btn-main\">Book Free Demo \u2192<\/a>\n      <a href=\"#cyt-example\" class=\"ica-btn-ghost\">See Practical Example \u2193<\/a>\n    <\/div>\n    <div class=\"ica-hero-full-stats\">\n      <div class=\"ica-hstat\"><div class=\"ica-hstat-n\">24<\/div><div class=\"ica-hstat-l\">conditions in parallel<\/div><\/div>\n      <div class=\"ica-hstat\"><div class=\"ica-hstat-n\">24\/7<\/div><div class=\"ica-hstat-l\">continuous monitoring<\/div><\/div>\n      <div class=\"ica-hstat\"><div class=\"ica-hstat-n\">10 min<\/div><div class=\"ica-hstat-l\">imaging interval<\/div><\/div>\n      <div class=\"ica-hstat\"><div class=\"ica-hstat-n\">0<\/div><div class=\"ica-hstat-l\">labels required<\/div><\/div>\n    <\/div>\n  <\/div>\n<\/div>\n\n\n<!-- \u2550\u2550\u2550 2. WHAT IS A CYTOTOXICITY ASSAY \u2550\u2550\u2550 -->\n<div class=\"ica-feat-full ica-sec-full\">\n  <div class=\"ica-kicker\">Background<\/div>\n  <h2>What Is a <span>Cytotoxicity Assay?<\/span><\/h2>\n\n  <div class=\"app-feature-row\" style=\"margin-top:2rem\">\n    <div class=\"app-feature-text\">\n      <h3>The Fundamental Drug Discovery Tool<\/h3>\n      <p>Cytotoxicity assays measure the degree to which a substance damages or destroys cells. They are a fundamental tool in drug discovery, toxicology testing and cancer research \u2014 used to predict tissue-specific toxicity and identify leads for anti-cancer therapies.<\/p>\n      <p>Traditional endpoint assays (MTT, LDH, Trypan Blue) provide a single snapshot at one time point. zenCELL owl replaces this with continuous brightfield imaging \u2014 capturing every morphological change from first drug treatment through to cell death.<\/p>\n      <ul>\n        <li>No labels, no staining, no cell lysis required<\/li>\n        <li>Kinetic data across the full assay duration<\/li>\n        <li>Morphology changes visible at single-cell level<\/li>\n        <li>Compare up to 24 drug conditions simultaneously<\/li>\n      <\/ul>\n    <\/div>\n    <div class=\"app-img-wrap\">\n      <img decoding=\"async\" src=\"https:\/\/zencellowl.com\/wp-content\/uploads\/2020\/03\/Cytotoxicity-Assay.png\" alt=\"Cytotoxicity assay live cell imaging with zenCELL owl incubator microscope\" class=\"app-img\" loading=\"eager\">\n    <\/div>\n  <\/div>\n<\/div>\n\n\n<!-- \u2550\u2550\u2550 3. HOW ZENCELL OWL HELPS \u2550\u2550\u2550 -->\n<div class=\"ica-pain-full ica-sec-full\" style=\"background:#f0f9f8\">\n  <div class=\"ica-kicker\">Why zenCELL owl<\/div>\n  <h2>Continuous Kinetics \u2014 <span>Not Just an Endpoint<\/span><\/h2>\n  <p class=\"ica-intro\">Standard cytotoxicity assays miss the full picture. zenCELL owl captures every time point \u2014 so you see when drug effects start, how they progress, and at what concentration they become lethal.<\/p>\n\n  <div class=\"app-adv-grid\">\n    <div class=\"ica-feat-full-card star\">\n      <span class=\"icon\">\ud83d\udcca<\/span>\n      <div class=\"title\">Full Kinetic Profile<\/div>\n      <p class=\"desc\">Image every 10 minutes throughout the entire assay. Capture onset of growth inhibition, morphological changes, and cell death \u2014 at every concentration and every time point retrospectively.<\/p>\n    <\/div>\n    <div class=\"ica-feat-full-card star\">\n      <span class=\"icon\">\ud83d\udd04<\/span>\n      <div class=\"title\">24 Conditions Simultaneously<\/div>\n      <p class=\"desc\">Run 24 drug concentrations, compounds or cell lines in parallel under identical incubator conditions. Dose-response relationships in a single experiment without sequential testing.<\/p>\n    <\/div>\n    <div class=\"ica-feat-full-card star\">\n      <span class=\"icon\">\ud83c\udfe0<\/span>\n      <div class=\"title\">Cells Stay in the Incubator<\/div>\n      <p class=\"desc\">No cell removal for imaging. No temperature or CO\u2082 fluctuations. No contamination risk from repeated handling. Environmental conditions remain stable throughout the entire assay.<\/p>\n    <\/div>\n    <div class=\"ica-feat-full-card\">\n      <span class=\"icon\">\ud83d\udd2c<\/span>\n      <div class=\"title\">Single-Cell Morphology<\/div>\n      <p class=\"desc\">5MP brightfield resolution captures granulation changes, cell rounding, detachment and death at single-cell level. Document drug mechanisms with timelapse videos.<\/p>\n    <\/div>\n    <div class=\"ica-feat-full-card\">\n      <span class=\"icon\">\ud83d\udce1<\/span>\n      <div class=\"title\">Remote Monitoring<\/div>\n      <p class=\"desc\">Check confluence and morphology from your PC or tablet \u2014 without entering the lab. Set confluence alerts and get notified when critical thresholds are reached.<\/p>\n    <\/div>\n    <div class=\"ica-feat-full-card\">\n      <span class=\"icon\">\ud83e\uddea<\/span>\n      <div class=\"title\">Label-Free Detection<\/div>\n      <p class=\"desc\">Brightfield imaging requires no fluorescent labels, no staining, no cell lysis. Cells remain viable and undisturbed. Combine with endpoint assays if needed \u2014 the cells are still intact.<\/p>\n    <\/div>\n  <\/div>\n<\/div>\n\n\n<!-- \u2550\u2550\u2550 4. PRACTICAL EXAMPLE \u2550\u2550\u2550 -->\n<div class=\"ica-vs-full ica-sec-full\" id=\"cyt-example\">\n  <div class=\"ica-kicker\">Practical example<\/div>\n  <h2>L929 Mouse Fibroblasts \u2014 <span>CAA &amp; DOX Treatment<\/span><\/h2>\n  <p class=\"ica-intro\">L929 mouse fibroblast cell cultures were treated with different concentrations of the cytostatic drugs Chloroacetaldehyde (CAA) and Doxorubicin (DOX). zenCELL owl captured the full kinetic dose-effect relationship for both drugs.<\/p>\n\n  <!-- Untreated vs treated -->\n  <div class=\"app-feature-row\" style=\"margin-top:2rem\">\n    <div>\n      <div style=\"display:grid;grid-template-columns:1fr 1fr;gap:12px\">\n        <div class=\"app-img-wrap\">\n          <img decoding=\"async\" src=\"https:\/\/zencellowl.com\/wp-content\/uploads\/2020\/03\/L929-fibroblasr-cell-line-mouse-recording-after-48-hours.png\" alt=\"L929 fibroblast untreated control \u2014 zenCELL owl brightfield imaging\" class=\"app-img\" loading=\"lazy\">\n          <p class=\"app-img-caption\">Untreated control \u2014 L929 cells at 48h<\/p>\n        <\/div>\n        <div class=\"app-img-wrap\">\n          <img decoding=\"async\" src=\"https:\/\/zencellowl.com\/wp-content\/uploads\/2020\/03\/L929-fibroblasr-cell-line-mouse-recording-after-48-hours.png\" alt=\"L929 fibroblast cytostatic drug treatment \u2014 morphology changes\" class=\"app-img\" loading=\"lazy\">\n          <p class=\"app-img-caption\">Cytostatic drug treatment \u2014 morphology loss<\/p>\n        <\/div>\n      <\/div>\n    <\/div>\n    <div class=\"app-feature-text\">\n      <h3>Dose-Dependent Morphology Changes<\/h3>\n      <p>Drug treatment results in growth inhibition, loss of the original cell morphology and changes of intracellular granulation. Finally, drug treatment results in cell death.<\/p>\n      <p>A time-dependent dose-effect relationship was observed for both cytostatic drugs \u2014 captured automatically by zenCELL owl without a single manual imaging step.<\/p>\n      <ul>\n        <li>Growth inhibition clearly visible in confluence curves<\/li>\n        <li>Morphological changes at single-cell level<\/li>\n        <li>Intracellular granulation changes captured<\/li>\n        <li>Time-dependent dose-effect relationship quantified<\/li>\n      <\/ul>\n    <\/div>\n  <\/div>\n\n  <!-- CAA and DOX images -->\n  <div class=\"app-example-grid\" style=\"margin-top:2rem\">\n    <div class=\"app-example-card\">\n      <img decoding=\"async\" src=\"https:\/\/zencellowl.com\/wp-content\/uploads\/2020\/03\/CAA-Effects-on-Cell-Morphology-1.png\" alt=\"CAA chloroacetaldehyde effects on L929 cell morphology \u2014 dose-dependent\" loading=\"lazy\">\n      <div class=\"app-example-body\">\n        <span class=\"tag\">CAA \u2014 Chloroacetaldehyde<\/span>\n        <p>Dose-dependent growth inhibition, loss of cell morphology and cell death. Digital phase-contrast imaging of L929 cells. Scalebar: 200 \u00b5m.<\/p>\n      <\/div>\n    <\/div>\n    <div class=\"app-example-card\">\n      <img decoding=\"async\" src=\"https:\/\/zencellowl.com\/wp-content\/uploads\/2020\/03\/asdsd.png\" alt=\"DOX doxorubicin effects on L929 cell morphology \u2014 growth inhibition\" loading=\"lazy\">\n      <div class=\"app-example-body\">\n        <span class=\"tag\">DOX \u2014 Doxorubicin<\/span>\n        <p>Growth inhibition, loss of cell morphology and cell death. Digital phase-contrast imaging of L929 cells. Scalebar: 200 \u00b5m.<\/p>\n      <\/div>\n    <\/div>\n  <\/div>\n\n  <!-- Application Note download -->\n  <div style=\"background:#edf6f5;border:1px solid rgba(58,174,160,.2);border-radius:14px;padding:20px 24px;margin-top:2rem;display:flex;align-items:center;gap:16px;flex-wrap:wrap\">\n    <div style=\"font-size:2rem;flex-shrink:0\">\ud83d\udcc4<\/div>\n    <div style=\"flex:1\">\n      <p style=\"font-size:14px;font-weight:700;color:#1a2e3a;margin:0 0 4px;font-family:'Montserrat',sans-serif\">Application Note \u2014 Cytotoxicity Assay<\/p>\n      <p style=\"font-size:13px;color:#5a6a7a;margin:0;font-family:'Montserrat',sans-serif\">Analysis of cytostatic drug effects with zenCELL owl. Full protocol, data and methodology.<\/p>\n    <\/div>\n    <a href=\"https:\/\/zencellowl.com\/wp-content\/uploads\/2023\/02\/zencell-owl_Analysis-of-Cytostatic-Drug-Effects.pdf\" target=\"_blank\" style=\"display:inline-block;background:#3aaea0;color:#fff;padding:11px 22px;border-radius:10px;font-family:'Montserrat',sans-serif;font-size:13px;font-weight:700;text-decoration:none;white-space:nowrap;flex-shrink:0\">Download PDF \u2192<\/a>\n  <\/div>\n<\/div>\n\n\n<!-- \u2550\u2550\u2550 5. PUBLICATIONS \u2550\u2550\u2550 -->\n<div class=\"ica-pub-full\">\n  <div class=\"ica-pub-full-num\">50+<\/div>\n  <div class=\"ica-pub-full-label\">peer-reviewed publications cite zenCELL owl \u2014 including cytotoxicity and drug effect studies<\/div>\n  <a href=\"https:\/\/zencellowl.com\/publication\/\" class=\"ica-pub-full-link\">View all publications \u2192<\/a>\n<\/div>\n\n\n<!-- \u2550\u2550\u2550 6. FAQ \u2550\u2550\u2550 -->\n<div class=\"ica-faq-full ica-sec-full\">\n  <div class=\"ica-kicker\">FAQ<\/div>\n  <h2>Cytotoxicity Assay \u2014 <span>Common Questions<\/span><\/h2>\n  <div class=\"ica-faq-full-list\">\n\n    <div class=\"ica-faq-full-item\">\n      <div class=\"ica-faq-full-q\" onclick=\"toggleFaqFull(this)\">What is the advantage of live cell imaging over MTT or LDH assays for cytotoxicity?<span class=\"ica-faq-full-ico\">+<\/span><\/div>\n      <div class=\"ica-faq-full-a\"><div style=\"padding:0 22px 18px;font-size:14px;color:#5a6a7a;line-height:1.7\">MTT and LDH assays provide a single endpoint measurement \u2014 you only see the result at one time point and the cells must be lysed or fixed. zenCELL owl delivers continuous kinetic data throughout the entire assay. You capture the full dose-effect relationship over time, observe morphology changes at single-cell level, and the cells remain alive and undisturbed throughout. Endpoint assays can then be added on top if needed.<\/div><\/div>\n    <\/div>\n\n    <div class=\"ica-faq-full-item\">\n      <div class=\"ica-faq-full-q\" onclick=\"toggleFaqFull(this)\">How many drug conditions can be tested in parallel?<span class=\"ica-faq-full-ico\">+<\/span><\/div>\n      <div class=\"ica-faq-full-a\"><div style=\"padding:0 22px 18px;font-size:14px;color:#5a6a7a;line-height:1.7\">Up to 24 conditions simultaneously in a single zenCELL owl \u2014 different drugs, different concentrations, different cell lines, or combinations. All 24 wells are imaged independently under identical incubator conditions. With the zenCELL owl SCAN add-on, this extends to full 96-well or 384-well plates.<\/div><\/div>\n    <\/div>\n\n    <div class=\"ica-faq-full-item\">\n      <div class=\"ica-faq-full-q\" onclick=\"toggleFaqFull(this)\">Does zenCELL owl require fluorescent labels for cytotoxicity assays?<span class=\"ica-faq-full-ico\">+<\/span><\/div>\n      <div class=\"ica-faq-full-a\"><div style=\"padding:0 22px 18px;font-size:14px;color:#5a6a7a;line-height:1.7\">No. zenCELL owl uses brightfield (transmitted light) imaging \u2014 no fluorescent labels, no staining, no cell lysis required. Cytotoxicity is assessed through morphology changes and confluence reduction, which are clearly visible in brightfield. This means cells remain alive and intact throughout the assay.<\/div><\/div>\n    <\/div>\n\n    <div class=\"ica-faq-full-item\">\n      <div class=\"ica-faq-full-q\" onclick=\"toggleFaqFull(this)\">What cell lines have been validated for cytotoxicity assays with zenCELL owl?<span class=\"ica-faq-full-ico\">+<\/span><\/div>\n      <div class=\"ica-faq-full-a\"><div style=\"padding:0 22px 18px;font-size:14px;color:#5a6a7a;line-height:1.7\">L929 mouse fibroblasts, MCF7 breast cancer cells, HeLa, CHO, HEK293 and many others have been used in published cytotoxicity studies with zenCELL owl. The system works with any adherent cell line that can be grown in standard cell culture vessels. Over 50 peer-reviewed publications document its use across a wide range of applications.<\/div><\/div>\n    <\/div>\n\n    <div class=\"ica-faq-full-item\">\n      <div class=\"ica-faq-full-q\" onclick=\"toggleFaqFull(this)\">Can I see a cytotoxicity assay running live before purchasing?<span class=\"ica-faq-full-ico\">+<\/span><\/div>\n      <div class=\"ica-faq-full-a\"><div style=\"padding:0 22px 18px;font-size:14px;color:#5a6a7a;line-height:1.7\">Yes \u2014 our free remote demos run twice per week via MS Teams. We show zenCELL owl live inside a real incubator with real cells. You can see the confluence tracking, morphology analysis and software interface in action. Book your free slot below.<\/div><\/div>\n    <\/div>\n\n  <\/div>\n<\/div>\n\n\n<!-- \u2550\u2550\u2550 7. CTA \u2550\u2550\u2550 -->\n<div class=\"ica-cta-full\">\n  <div class=\"ica-cta-full-in\">\n    <h2>See Cytotoxicity Monitoring Live \u2014<br><span>Free Remote Demo<\/span><\/h2>\n    <p class=\"ica-cta-full-text\">Watch zenCELL owl track drug effects on real cells inside a real incubator. Twice per week via MS Teams. No obligation.<\/p>\n    <div class=\"ica-cta-full-btns\">\n      <a href=\"https:\/\/zencellowl.com\/live-remotedemo\/\" class=\"ica-btn-white\">Book Free Demo \u2192<\/a>\n      <a href=\"https:\/\/zencellowl.com\/cell-imaging\/\" class=\"ica-btn-outline\">zenCELL owl System<\/a>\n    <\/div>\n    <div class=\"ica-cta-full-trust\">\n      <span>Free via MS Teams<\/span>\n      <span>Twice per week<\/span>\n      <span>No commitment<\/span>\n      <span>50+ publications<\/span>\n    <\/div>\n  <\/div>\n<\/div>\n\n\n<!-- \u2550\u2550\u2550 RELATED APPLICATIONS \u2550\u2550\u2550 -->\n<div style=\"background:#f8fafa;padding:24px 0;border-top:1px solid #e8ecee\">\n  <div style=\"max-width:1140px;margin:0 auto;padding:0 2rem;display:flex;align-items:center;gap:12px;flex-wrap:wrap\">\n    <span style=\"font-size:.78rem;font-weight:700;color:#7f8c8d;text-transform:uppercase;letter-spacing:.1em;flex-shrink:0;font-family:'Montserrat',sans-serif\">Other applications:<\/span>\n    <a href=\"https:\/\/zencellowl.com\/cell-culture-monitoring\/\" style=\"font-size:.82rem;font-weight:600;color:#3aaea0;text-decoration:none;padding:5px 12px;border-radius:100px;border:1px solid rgba(58,174,160,.2);background:rgba(58,174,160,.05);font-family:'Montserrat',sans-serif\">Cell Culture Monitoring<\/a>\n    <a href=\"https:\/\/zencellowl.com\/scratch-assay\/\" style=\"font-size:.82rem;font-weight:600;color:#3aaea0;text-decoration:none;padding:5px 12px;border-radius:100px;border:1px solid rgba(58,174,160,.2);background:rgba(58,174,160,.05);font-family:'Montserrat',sans-serif\">Scratch Assay<\/a>\n    <a href=\"https:\/\/zencellowl.com\/zencell-spheroid\/\" style=\"font-size:.82rem;font-weight:600;color:#3aaea0;text-decoration:none;padding:5px 12px;border-radius:100px;border:1px solid rgba(58,174,160,.2);background:rgba(58,174,160,.05);font-family:'Montserrat',sans-serif\">Spheroid Imaging<\/a>\n    <a href=\"https:\/\/zencellowl.com\/cell-intoxication\/\" style=\"font-size:.82rem;font-weight:600;color:#3aaea0;text-decoration:none;padding:5px 12px;border-radius:100px;border:1px solid rgba(58,174,160,.2);background:rgba(58,174,160,.05);font-family:'Montserrat',sans-serif\">Cell Intoxication<\/a>\n    <a href=\"https:\/\/zencellowl.com\/hypoxic-cell-culture-monitoring\/\" style=\"font-size:.82rem;font-weight:600;color:#3aaea0;text-decoration:none;padding:5px 12px;border-radius:100px;border:1px solid rgba(58,174,160,.2);background:rgba(58,174,160,.05);font-family:'Montserrat',sans-serif\">Hypoxic Monitoring<\/a>\n    <a href=\"https:\/\/zencellowl.com\/cell-imaging\/\" style=\"font-size:.82rem;font-weight:600;color:#3aaea0;text-decoration:none;padding:5px 12px;border-radius:100px;border:1px solid rgba(58,174,160,.2);background:rgba(58,174,160,.05);font-family:'Montserrat',sans-serif\">zenCELL owl System<\/a>\n  <\/div>\n<\/div>\n\n<\/div><!-- end .ica-full -->\n\n<script type=\"application\/ld+json\">\n{\"@context\":\"https:\/\/schema.org\",\"@type\":\"FAQPage\",\"mainEntity\":[\n{\"@type\":\"Question\",\"name\":\"What is the advantage of live cell imaging over MTT or LDH assays for cytotoxicity?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"MTT and LDH give a single endpoint \u2014 cells must be lysed. zenCELL owl delivers continuous kinetic data throughout the full assay. You capture dose-effect relationships over time, observe morphology at single-cell level, and cells remain alive throughout.\"}},\n{\"@type\":\"Question\",\"name\":\"How many drug conditions can be tested in parallel with zenCELL owl?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"Up to 24 conditions simultaneously \u2014 different drugs, concentrations or cell lines. With the SCAN add-on, extends to full 96-well or 384-well plates.\"}},\n{\"@type\":\"Question\",\"name\":\"Does zenCELL owl require fluorescent labels for cytotoxicity assays?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"No. Brightfield imaging only \u2014 no labels, no staining, no cell lysis. Cytotoxicity assessed through morphology changes and confluence reduction.\"}},\n{\"@type\":\"Question\",\"name\":\"What cell lines have been validated for cytotoxicity assays with zenCELL owl?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"L929 mouse fibroblasts, MCF7, HeLa, CHO, HEK293 and many others. 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drug treatment<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>\ud83d\udc8a Application \u2014 Cytotoxicity Assay Cytotoxicity Assay \u2014Kinetic, Label-Free, 24 Wells in Parallel Monitor morphological drug effects on up to 24 cell cultures simultaneously \u2014 24\/7 inside the incubator. No [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":1027,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"_acf_changed":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-317","page","type-page","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Cytotoxicity Assay with Live Cell Imaging | zenCELL owl<\/title>\n<meta name=\"description\" content=\"Monitor drug effects on 24 cell cultures simultaneously \u2014 24\/7, label-free, kinetic. No endpoint assay. Free demo. zenCELL owl from 14,000 \u20ac.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/zencellowl.com\/de\/cytotoxicity-assay\/\" \/>\n<meta property=\"og:locale\" content=\"de_DE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Cytotoxicity Assay with Live Cell Imaging | zenCELL owl\" \/>\n<meta property=\"og:description\" content=\"Monitor drug effects on 24 cell cultures simultaneously \u2014 24\/7, label-free, kinetic. No endpoint assay. 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