Application Note: Cytostatic Drug Effects

Application Note: Cytostatic Drug Effects

Analysis of Cytostatic Drug Effects using the 24-channel microscope zenCELL owl

Untreated L929 fibroblast cell line growth behavior

A vast amount of cell divisions result in the formation of a confluent cell monolayer of the untreated L929 cell line over a time period of 28 hours. In order to perform a cell division, fibroblast cells detach from the substrate surface, divide into daughter cells and attach back to the substrate.

L929 fibroblast cell line treated with the cytostatic drug Chloroacetaldehyde

Treatment with the cytostatic drug Chloroacetaldehyde [50 µM] leads to inhibition of cell proliferation and visible morphologic changes, detachment from the surface and ultimate cell death. Video shows a time period of 12 hours.

CAA effects on cell count

Dose-dependent decline of cell count. Standardized mean values for each condition are shown. n=8 for all groups.

CAA effects on cell coverage

Standardized mean values for each condition are shown. n=8 for all groups.

CAA effects on cell morphology

CAA treatment results in a dose-dependent growth inhibition, loss of cell morphology and cell death. Digital phase-contrast imaging of L929 cells. Scalebar: 200 µm

DOX effects on cell count

Changes in cell count resulting from DOX treatment. Standardized mean values for each condition are shown. n=8 for all groups.

DOX effects on cell coverage

Standardized mean values for each condition are shown. n=8 for all groups.

DOX effects on cell morphology

DOX treatment results in growth inhibition, loss of cell morphology and cell death. Digital phase-contrast imaging of L929 cells. Scalebar: 200 µm.