Documentation of cell culture quality

Application Note: Documentation of cell culture quality

Documentation of cell culture quality using the 24-channel microscope zenCELL owl

In order to conduct reproducible, significant and objective exper-iments it is essential to ensure consistent cell culture quality. The base for standardizable analysis is formed by defined and stable environmental conditions including temperature, CO2-supply and the humidity inside the cell incubator. To ensure comparability of growth conditions it is necessary to use standardized processes, defined by Standard Operating Procedures (SOP).

zenCELL owl. 24-channel microscope with 24-well cell culture plate.

Documentation of culture quality during growth phase

24-Well cell culture plate 34 hours after seeding of L929 Fibroblasts

a) Overview
zenCELL owl software window “Overview” provides a direct comparison of the cell density and vitality of all 24 wells in parallel.

b) Well History
zenCELL owl software window “Well History” shows the parameter cell coverage, adherent cell number, cell morphology for each single culture well in detail.

Documentation of culture quality for subsequent experiments at the end of cell culture phase

24-Well cell culture plate 86 hours after seeding of L929 Fibroblasts

a) Overview
zenCELL owl software window “Overview” provides a direct comparison of the cell density and vitality of all 24 wells in parallel.

b) Well History
zenCELL owl software window “Well History” shows the parameter cell coverage, adherent cell number, cell morphology for each single culture well in detail.