{"id":321,"date":"2020-03-02T13:13:19","date_gmt":"2020-03-02T12:13:19","guid":{"rendered":"https:\/\/zencellowl.com\/?page_id=321"},"modified":"2026-05-10T16:24:18","modified_gmt":"2026-05-10T14:24:18","slug":"scratch-assay","status":"publish","type":"page","link":"https:\/\/zencellowl.com\/es\/scratch-assay\/","title":{"rendered":"Ensayo de Rayado"},"content":{"rendered":"
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\n\t\t\t\t\tScratch assay<\/span>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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zenCELL owl<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Record every movement. Calculate speed accurately.<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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aplicaciones zenCELL owl<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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The wound healing, migration assay or scratch assay is a standard method to analyze cell migration in vitro<\/i>. The dynamics of cell migration into a cell-free area, which has been created by direct manipulation or physical exclusion, are monitored and quantified to analyze cell migration characteristics and to calculate the gap closure time.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Our Process<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Dynamic data acquisition for dynamic processes<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\t\t\tWith conventional manual microscopy it is not possible to analyze each dynamic change which happens on cell levels at any time. zenCELL owl records pictures in an intervals of 5 minutes and every cell movement in detail \u2013 also during long-term analyses.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Wound healing speed - accurately calculated<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\t\t\tObserve and control every time-point and step of wound healing process retrospectively and in detail. Calculate the speed of wound healing by analyzing the increase of confluence in the gap area.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Parallelize 24 migration assays under identical conditions<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\t\t\tThe simultaneous analysis of cell morphology and confluence in up to 24 wells under identical conditions optimizes the comparability of your results and reproducibility of data.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\tShow me more about zenCELL owl<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Practical example<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Scratch assay with zenCELL owl<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Visualize and analyze the process of gap closure<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\t\t\tA migration assay of L929 fibroblasts were performed over a time period of 24 hours. The gap was inserted in a confluent monolayer by a pipet tip (0 hours). 1 hour after inserting the gap cells on the edge start to migrate. Within a time-period of 24 hours the cell migration results in a continuous closure of the gap.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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\"Close-up<\/figure><\/div>
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Calculate the speed of gap closure<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Data of Cell Coverage can be used to calculate the \u201cRelative Gap Area\u201d manually. Plotted as a function of time, the time-point of half gap closure (t1\/2 Gap Closure) can be determined from this graph.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Scratch assay - Application Note<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\tDownload<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

Scratch assay zenCELL owl Record every movement. Calculate speed accurately. https:\/\/zencellowl.com\/wp-content\/uploads\/2020\/03\/Scratch-Assay.mp4 zenCELL owl applications The wound healing, migration assay or scratch assay is a standard method to analyze cell migration […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"_acf_changed":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-321","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"\nAutomated Scratch Assay Microscope | zenCELL owl<\/title>\n<meta name=\"description\" content=\"Run 24 wound healing assays in parallel \u2013 fully automated. 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