{"id":4027,"date":"2025-06-20T12:17:01","date_gmt":"2025-06-20T10:17:01","guid":{"rendered":"https:\/\/zencellowl.com\/?page_id=4027"},"modified":"2025-06-28T20:04:35","modified_gmt":"2025-06-28T18:04:35","slug":"cell-intoxication","status":"publish","type":"page","link":"https:\/\/zencellowl.com\/es\/cell-intoxication\/","title":{"rendered":"Intoxicaci\u00f3n celular"},"content":{"rendered":"
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Application: Monitoring Bacterial Toxin Activity via Morphological Cell Changes<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Real-time cell behavior reveals toxin effects \u2013 with precision and ease<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Bacterial toxins, such as those produced by Clostridioides difficile, play a key role in diseases like
pseudomembranous colitis. Once internalized, these toxins enzymatically disrupt cellular signaling and
morphology \u2013 visible through distinct shape changes in adherent cells.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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This application highlights the use of zenCELL owl to monitor cell intoxication in real time, using
morphological changes as a specific readout for toxin activity.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Montaje experimental<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\t\t\tSe llevaron a cabo dos enfoques experimentales:\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Objective: Visualize and quantify intoxication kinetics of Vero cells exposed to C. difficile TcdA toxin.<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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  • \n\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t<\/i>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\tCell model<\/b> Vero cells (African green monkey kidney epithelial cells)<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t
  • \n\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t<\/i>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\tAssay setup<\/b> 24-well plates, incubated at 37\u00b0C<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t
  • \n\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t<\/i>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\tTreatment<\/b> TcdA at 5 ng\/mL<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t
  • \n\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t<\/i>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\tImaging interval<\/b> Every 30 minutes for 15 hours<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t
  • \n\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t<\/i>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\tMicroscope<\/b> zenCELL owl live-cell incubator microscope<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t<\/ul>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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    \n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\"TcdA\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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    Morphological Monitoring of Cell Intoxication<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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    Within the first hour, cells exposed to TcdA begin to round up<\/strong> \u2013 a hallmark morphological change
    indicating Rho protein glucosylation and cytoskeletal collapse.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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    Time-lapse observations:<\/strong> – 1 h<\/strong>: Early onset of cell rounding – 3 h<\/strong>: Increased frequency of rounded
    cells – 8 h<\/strong>: Widespread intoxication across the well<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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    \n\t\t\t\t\t\t\t\t\tThese effects were continuously documented using zenCELL owl, providing high-resolution, noninvasive tracking of toxin-induced morphology changes.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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    \n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\"Cell\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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    Why zenCELL owl?\n<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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    \n\t\t\t\t\t\tOperates directly in the incubator \u2013 no disturbance to conditions\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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    \n\t\t\t\t\t\t 24-camera setup for parallel sample observation\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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    \n\t\t\t\t\t\tAutomated time-lapse imaging and analysis\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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    \n\t\t\t\t\t\t Easy-to-use hardware and intuitive software\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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    \n\t\t\t\t\t\tIdeal for monitoring cytotoxicity, drug response & functional assays\n\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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    Conclusi\u00f3n<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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    \n\t\t\t\t\t\t\t\t\tzenCELL owl streamlines morphological assays by combining incubation-stable monitoring\nwith real-time image acquisition. Perfect for studying dynamic processes like bacterial intoxication with\nminimal lab intervention.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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    Developed by:<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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    Institute of Pharmacology and Toxicology, Ulm University Medical Center<\/strong>
    Authors: Maria Braune, Katharina Ernst<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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    Explore zenCELL owl\n<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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    Learn more about our compact 24-channel incubator microscope for real-time, reproducible, and automated cell culture monitoring:<\/p>

    www.zencellowl.com
    Follow us on LinkedIn: zenCELL owl \/ innoME GmbH<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

    Application: Monitoring Bacterial Toxin Activity via Morphological Cell Changes Real-time cell behavior reveals toxin effects \u2013 with precision and ease Bacterial toxins, such as those produced by Clostridioides difficile, play […]<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"_acf_changed":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-4027","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"\nCell Intoxication Assay \u2013 Real-Time Monitoring of Toxin Effects<\/title>\n<meta name=\"description\" content=\"Monitor cell intoxication in real time. 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