{"id":7187,"date":"2026-08-22T22:54:03","date_gmt":"2026-08-22T20:54:03","guid":{"rendered":"https:\/\/zencellowl.com\/?page_id=7187"},"modified":"2026-08-22T23:25:31","modified_gmt":"2026-08-22T21:25:31","slug":"wound-healing-assay-without-fluorescence","status":"publish","type":"page","link":"https:\/\/zencellowl.com\/zh\/wound-healing-assay-without-fluorescence\/","title":{"rendered":"Why do some wound healing assays require fluorescence?"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"7187\" class=\"elementor elementor-7187\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-dfe7c6a e-flex e-con-boxed e-con e-parent\" data-id=\"dfe7c6a\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-c1ee379 elementor-widget elementor-widget-html\" data-id=\"c1ee379\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"html.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p>ScratchMaker Plates enable standardized wound healing assays using brightfield microscopy only \u2014 no fluorescence microscope required. This is the key differentiator from insert-based methods (ibidi) and many commercial wound healing solutions that assume fluorescence capability. Any inverted brightfield microscope, or an in-incubator brightfield imager like zenCELL owl, is sufficient.<\/p>\n\n<hr>\n\n<h2>Do I need a fluorescence microscope for a wound healing assay?<\/h2>\n<p>No. The wound healing assay and scratch assay measure cell-free area closure over time. Cell-free area is directly visible in brightfield (transmitted white light) \u2014 no staining, no fluorescent labels, no fluorescence excitation required. ScratchMaker Plates create a defined cell-free wound using UV-A photochemical treatment before seeding or after monolayer formation. The resulting wound is imaged in brightfield at T=0 and subsequent timepoints.<\/p>\n\n<h2>Why do some wound healing assays require fluorescence?<\/h2>\n<p>Insert-based systems (e.g. ibidi Culture-Insert) leave a physical gap after insert removal. This gap is visible in brightfield. However, some researchers use fluorescent cell labelling to improve contrast in brightfield-challenging conditions (highly transparent cells, low cell density). This is a workaround for brightfield imaging limitations \u2014 not a fundamental requirement of the wound healing assay itself. ScratchMaker Plates produce high-contrast wounds visible in standard brightfield without any labelling.<\/p>\n\n<h2>What microscopes are compatible with ScratchMaker Plates?<\/h2>\n<ul>\n<li>Any standard inverted brightfield microscope (Zeiss, Leica, Olympus, Nikon, Keyence)<\/li>\n<li>zenCELL owl in-incubator imager \u2014 automated 24-well simultaneous imaging<\/li>\n<li>Phase contrast microscopes<\/li>\n<li>Standard smartphone microscopy adapters (for basic documentation)<\/li>\n<\/ul>\n<p>A fluorescence light source, fluorescence objectives, or fluorescence filters are not needed.<\/p>\n\n<h2>How does ScratchMaker create the wound without a pipette tip?<\/h2>\n<p>ScratchMaker Plates have a photosensitizer coating applied to the glass bottom. A reusable precision UV-A light mask is placed over the plate and exposed to UV-A light. The photosensitizer absorbs UV-A energy and generates reactive oxygen species that kill cells in a precisely defined strip \u2014 typically 300\u2013800 \u00b5m wide depending on the mask design. Surrounding cells are unaffected. The resulting wound is defined by the mask geometry, not by operator technique.<\/p>\n\n<h2>What is the wound width and how reproducible is it?<\/h2>\n<p>ScratchMaker wound width is defined by the UV-A light mask geometry. Coefficient of variation (CV) across wells and plates is below 5%. In comparison, pipette tip scratching produces CV values of 30\u201360% across operators. The wound position is fixed relative to the well bottom \u2014 the same field of view captures the wound at every imaging timepoint without repositioning.<\/p>\n\n<h2>Are ScratchMaker Plates compatible with ECM coatings?<\/h2>\n<p>Yes. ECM protein coatings (Fibronectin, Vitronectin, Collagen I, Laminin, Poly-L-Lysine) are applied to the glass bottom before cell seeding and UV-A exposure. The coating does not affect UV-A photochemical wound creation. ECM-coated ScratchMaker Plates are used for migration studies requiring specific integrin-ECM interactions.<\/p>\n\n<h2>What formats are available?<\/h2>\n<p>ScratchMaker Plates are available in 6-well, 24-well, and 96-well glass-bottom formats. The 96-well format is compatible with the zenCELL owl Scan (owl SCAN) for high-throughput screening \u2014 imaging a full 96-well plate in approximately 90 seconds.<\/p>\n\n<h2>What is the price?<\/h2>\n<p>ScratchMaker Starter Kit: \u20ac499 (includes plates and reusable light mask). Individual plates from \u20ac59. For high-volume users, contact innoME GmbH for volume pricing.<\/p>\n\n<table>\n<thead>\n<tr><th>Feature<\/th><th>ScratchMaker Plates<\/th><th>Pipette Tip Scratch<\/th><th>ibidi Insert<\/th><\/tr>\n<\/thead>\n<tbody>\n<tr><td>Wound width CV<\/td><td>&lt;5%<\/td><td>30\u201360%<\/td><td>&lt;10%<\/td><\/tr>\n<tr><td>Fluorescence required<\/td><td>No<\/td><td>No<\/td><td>No (but often used)<\/td><\/tr>\n<tr><td>Cell contact in wound<\/td><td>Yes \u2014 cells grow on coating<\/td><td>Yes<\/td><td>No \u2014 insert artefact<\/td><\/tr>\n<tr><td>ECM coating compatible<\/td><td>Yes<\/td><td>Partially<\/td><td>Limited<\/td><\/tr>\n<tr><td>96-well HTS format<\/td><td>Yes<\/td><td>No<\/td><td>No<\/td><\/tr>\n<tr><td>Cost per plate<\/td><td>From \u20ac59<\/td><td>~\u20ac1\u20132 (tip only)<\/td><td>~\u20ac80\u2013120<\/td><\/tr>\n<\/tbody>\n<\/table>\n\n<p><a href=\"https:\/\/zencellowl.com\/live-remotedemo\/\"><strong>Book a free demo \u2192<\/strong><\/a> or <a href=\"https:\/\/zencellowl.com\/scratchmaker-plates\/\"><strong>Order a Starter Kit \u2192<\/strong><\/a><\/p>\n\n<script type=\"application\/ld+json\">\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"FAQPage\",\n  \"mainEntity\": [\n    { \"@type\": \"Question\", \"name\": \"Do I need a fluorescence microscope for a wound healing assay?\", \"acceptedAnswer\": { \"@type\": \"Answer\", \"text\": \"No. Wound healing assays run entirely in brightfield \u2014 cell-free wound area is visible without staining or fluorescent labels. 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This is the key differentiator from insert-based methods (ibidi) and many commercial wound healing [&hellip;]<\/p>\n","protected":false},"author":7,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_monsterinsights_skip_tracking":false,"footnotes":""},"class_list":["post-7187","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Wound Healing Assay Without Fluorescence Microscope zencell<\/title>\n<meta name=\"description\" content=\"Run reproducible wound healing assays with any brightfield microscope \u2014 no fluorescence required. ScratchMaker Plates: CV &lt;5%, 96-well compatible. 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