{"id":7076,"date":"2026-07-26T18:16:41","date_gmt":"2026-07-26T16:16:41","guid":{"rendered":"https:\/\/zencellowl.com\/?p=7076"},"modified":"2026-07-26T18:16:41","modified_gmt":"2026-07-26T16:16:41","slug":"wound-healing-assay-guide","status":"publish","type":"post","link":"https:\/\/zencellowl.com\/zh\/wound-healing-assay-guide\/","title":{"rendered":"Wound Healing Assay \u2014 Complete Protocol &#038; Method Guide 2026"},"content":{"rendered":"<p><!-- BLOG ARTICLE \u2014 Wound Healing Assay Complete Guide --><br \/>\n<!-- Target: Page 1 for \"wound healing assay\", \"scratch assay protocol\", \"cell migration assay\" --><br \/>\n<!-- AEO optimized for People Also Ask + Featured Snippets --><br \/>\n<!-- Elementor HTML Block \u2014 zencellowl.com Design Standard --><\/p>\n<p><script type=\"application\/ld+json\">\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"FAQPage\",\n  \"mainEntity\": [\n    {\n      \"@type\": \"Question\",\n      \"name\": \"What is a wound healing assay?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"A wound healing assay is an in vitro method to measure two-dimensional cell migration. A gap is created in a confluent cell monolayer and cells are imaged over time as they migrate to close the wound. Also called a scratch assay or gap closure assay, it is used in cancer research, drug screening, and regenerative medicine to quantify cell motility and collective migration.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"What is the difference between a wound healing assay and a scratch assay?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"A wound healing assay and a scratch assay refer to the same basic experiment \u2014 creating a cell-free gap in a confluent monolayer and measuring how cells migrate to close it. The term 'scratch assay' refers specifically to the manual method using a pipette tip, while 'wound healing assay' is the broader category that includes photochemical, insert-based, and mechanical methods.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"How do you perform a wound healing assay?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"The standard wound healing assay protocol has 4 steps: (1) Seed cells in a well plate and grow to \u226595% confluency (16\u201324 h). (2) Create a wound \u2014 either by pipette tip (manual), silicone insert removal, or photochemical light exposure. (3) Image the wound at T=0. (4) Image at defined intervals (6h, 12h, 24h) and calculate wound closure percentage: (Initial area \u2212 Current area) \/ Initial area \u00d7 100.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"What is the most reproducible wound healing assay method?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Photochemical wound creation achieves the highest reproducibility with wound width CV below 5%, compared to \u00b130\u201360% for manual pipette scratching and \u00b115\u201325% for insert-based methods. A light mask clips under the plate and defines identical wound geometry in every well \u2014 independent of operator.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"How do you calculate wound closure in a scratch assay?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Wound closure (%) = (Initial wound area \u2212 Current wound area) \/ Initial wound area \u00d7 100. Measure wound area at T=0 and each subsequent timepoint using ImageJ (free Wound Healing Size Tool plugin) or automated software. Express results as % wound closure over time to generate a wound closure curve.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"What cells can be used in a wound healing assay?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Any adherent cell line can be used in a wound healing assay. Commonly used cell lines include HaCaT (keratinocytes), HUVECs (endothelial cells), A549 (lung cancer), MDA-MB-231 (breast cancer), U87-MG (glioblastoma), 3T3 fibroblasts, and primary cells. Cells must form a confluent monolayer (\u226595%) before wound creation.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Do I need a fluorescence microscope for a wound healing assay?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"No. A standard brightfield microscope is sufficient for wound healing assay imaging. A fluorescence microscope is only required if you are imaging fluorescently labelled cells. For photochemical wound creation with ScratchMaker plates, a ~395 nm light source (LED lamp, Hg-vapour DAPI channel, or 405 nm laser) is needed \u2014 not a full fluorescence microscope.\"\n      }\n    }\n  ]\n}\n<\/script><\/p>\n<style>\n  :root {<br \/>\n    --teal: #3aaea0;<br \/>\n    --navy: #1a2e3a;<br \/>\n    --white: #ffffff;<br \/>\n    --light: #f5f8f8;<br \/>\n    --lt: #e8f5f4;<br \/>\n    --bd: #e0eeec;<br \/>\n    --text: #222222;<br \/>\n    --sub: #555555;<br \/>\n    --red: #c62828;<br \/>\n    --green: #2e7d32;<br \/>\n    --font: 'Montserrat', sans-serif;<br \/>\n  }<br \/>\n  .art * { box-sizing: border-box; margin: 0; padding: 0; }<br \/>\n  .art { font-family: var(--font); color: var(--text); max-width: 860px; margin: 0 auto; padding: 0 24px 64px; }<\/p>\n<p>  .art h1 { font-family: var(--font); font-size: 32px; font-weight: 800; color: var(--navy); line-height: 1.25; margin: 32px 0 16px; }<br \/>\n  .art h2 { font-family: var(--font); font-size: 22px; font-weight: 800; 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font-size: 18px; font-weight: 800; color: white; margin-bottom: 6px; }<br \/>\n  .cta-box p { font-size: 14px; color: #d0e8ec; margin: 0; }<br \/>\n  .cta-box a { display: inline-block; background: white; color: var(--teal); font-family: var(--font); font-weight: 800; font-size: 14px; padding: 13px 24px; text-decoration: none; white-space: nowrap; flex-shrink: 0; }<br \/>\n  .cta-box a:hover { background: var(--navy); color: white; }<\/p>\n<p>  \/* NOTE BOX *\/<br \/>\n  .note-box { background: var(--light); border-left: 4px solid var(--teal); padding: 16px 20px; margin: 20px 0; }<br \/>\n  .note-box p { font-size: 14px; color: var(--text); margin: 0; line-height: 1.7; }<\/p>\n<p>  @media (max-width: 600px) {<br \/>\n    .art h1 { font-size: 24px; }<br \/>\n    .art h2 { font-size: 18px; }<br \/>\n    .steps-grid { grid-template-columns: 1fr 1fr; }<br \/>\n    .stat-strip { grid-template-columns: 1fr 1fr; }<br \/>\n    .cta-box { flex-direction: column; }<br \/>\n  }<br \/>\n<\/style>\n<div class=\"art\">\n<p><span class=\"art-eyebrow\">Cell Migration \u00b7 Wound Healing \u00b7 Scratch Assay<\/span><\/p>\n<h1>Wound Healing Assay \u2014 Complete Guide: Protocol, Methods &amp; Reproducibility<\/h1>\n<p>The wound healing assay \u2014 also called the scratch assay or gap closure assay \u2014 is one of the most widely used methods in cell biology to measure collective cell migration in vitro. This guide covers everything from the basic protocol to the most common sources of variability and how to eliminate them.<\/p>\n<p><!-- AEO ANSWER BOX --><\/p>\n<div class=\"answer-box\"><span class=\"al\">Quick Answer<\/span>A wound healing assay measures two-dimensional cell migration by creating a defined cell-free gap in a confluent monolayer and imaging how cells migrate to close it over time. Results are expressed as wound closure percentage over time. The key challenge is reproducibility: manual pipette scratching produces wound width variability of \u00b130\u201360%, making drug effects below this threshold statistically invisible.<\/p>\n<\/div>\n<h2>What is a Wound Healing Assay?<\/h2>\n<p>A wound healing assay is an in vitro method to study <strong>collective cell migration<\/strong> \u2014 how a sheet of cells responds to a gap and moves to fill it. <cite>It is specifically a 2D cell migration approach to semi-quantitatively measure cell migration of a sheet of cells.<\/cite> This is distinct from individual cell chemotaxis (Boyden chamber, Transwell assay) which measures single-cell movement through a membrane.<\/p>\n<p>The assay is used across a wide range of research areas:<\/p>\n<ul>\n<li><strong>Cancer biology<\/strong> \u2014 measuring tumour cell invasion, metastatic potential, and drug effects on migration<\/li>\n<li><strong>Drug screening<\/strong> \u2014 quantifying the effect of compounds on cell motility<\/li>\n<li><strong>Regenerative medicine<\/strong> \u2014 studying tissue repair mechanisms and potential therapeutics<\/li>\n<li><strong>Wound healing research<\/strong> \u2014 modelling re-epithelialisation and keratinocyte migration<\/li>\n<\/ul>\n<div class=\"stat-strip\">\n<div class=\"stat-item\">\n<div class=\"stat-val\">2D<\/div>\n<div class=\"stat-label\">Collective migration \u2014 sheet of cells, not individual<\/div>\n<\/div>\n<div class=\"stat-item\">\n<div class=\"stat-val\">24\u201372h<\/div>\n<div class=\"stat-label\">Typical experiment duration depending on cell type<\/div>\n<\/div>\n<div class=\"stat-item\">\n<div class=\"stat-val\">\u00b130\u201360%<\/div>\n<div class=\"stat-label\">Wound width CV with manual pipette scratching<\/div>\n<\/div>\n<div class=\"stat-item\">\n<div class=\"stat-val\">&lt;5%<\/div>\n<div class=\"stat-label\">Wound width CV with photochemical wound creation<\/div>\n<\/div>\n<\/div>\n<h2>Wound Healing Assay Protocol \u2014 Step by Step<\/h2>\n<p>The standard wound healing assay protocol follows four steps regardless of the wound creation method used:<\/p>\n<div class=\"steps-grid\">\n<div class=\"step-box\">\n<div class=\"step-num\">1<\/div>\n<h4>Seed &amp; Grow<\/h4>\n<p>Seed adherent cells at appropriate density. Incubate to \u226595% confluency \u2014 typically 16\u201324 h depending on cell line.<\/p>\n<\/div>\n<div class=\"step-box\">\n<div class=\"step-num\">2<\/div>\n<h4>Create Wound<\/h4>\n<p>Create a cell-free gap using pipette tip (manual), silicone insert removal, or photochemical light exposure.<\/p>\n<\/div>\n<div class=\"step-box\">\n<div class=\"step-num\">3<\/div>\n<h4>Image T=0<\/h4>\n<p>Image the wound immediately after creation. This is your reference wound area for all subsequent calculations.<\/p>\n<\/div>\n<div class=\"step-box\">\n<div class=\"step-num\">4<\/div>\n<h4>Monitor &amp; Quantify<\/h4>\n<p>Image at defined intervals. Calculate wound closure % at each timepoint.<\/p>\n<\/div>\n<\/div>\n<h3>Step 1 \u2014 Cell Seeding and Confluency<\/h3>\n<p>Seed cells at a density that reaches \u226595% confluency within 16\u201324 hours. <cite>A cell dose curve can be performed in a regular 24-well plate beforehand to find out how many cells are needed to form a monolayer within a certain time.<\/cite> Incomplete confluency leads to variable wound closure rates because non-migrating proliferating cells fill the gap instead of migrating cells.<\/p>\n<div class=\"note-box\">\n<p><strong>Critical:<\/strong> Do not move the plate unnecessarily during monolayer formation. Any disturbance causes uneven cell distribution that will affect wound geometry and closure rate.<\/p>\n<\/div>\n<h3>Step 2 \u2014 Wound Creation Methods<\/h3>\n<p>The choice of wound creation method is the single most important factor for reproducibility. Three methods are in common use:<\/p>\n<table class=\"comp-table\">\n<thead>\n<tr>\n<th>Method<\/th>\n<th class=\"teal\">ScratchMaker (Photochemical)<\/th>\n<th>Manual Pipette Scratch<\/th>\n<th>Insert-Based (ibidi)<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td class=\"cr\">Wound width CV<\/td>\n<td class=\"g\">&lt;5%<\/td>\n<td class=\"b\">\u00b130\u201360%<\/td>\n<td class=\"m\">\u00b115\u201325%<\/td>\n<\/tr>\n<tr>\n<td class=\"cr\">ECM coating after wounding<\/td>\n<td class=\"g\">Fully intact<\/td>\n<td class=\"b\">Physically removed<\/td>\n<td class=\"b\">Blocked beneath insert<\/td>\n<\/tr>\n<tr>\n<td class=\"cr\">Physiological wound model<\/td>\n<td class=\"g\">Yes \u2014 real cell death<\/td>\n<td class=\"b\">Mechanical rupture<\/td>\n<td class=\"b\">Artificial gap \u2014 no cell death<\/td>\n<\/tr>\n<tr>\n<td class=\"cr\">Washing step required<\/td>\n<td class=\"g\">Not required<\/td>\n<td class=\"b\">Recommended<\/td>\n<td class=\"b\">Required (insert removal)<\/td>\n<\/tr>\n<tr>\n<td class=\"cr\">96-well scalable<\/td>\n<td class=\"g\">Yes \u2014 fully automated<\/td>\n<td class=\"b\">Impractical<\/td>\n<td class=\"m\">Manual insert handling<\/td>\n<\/tr>\n<tr>\n<td class=\"cr\">Microscope required<\/td>\n<td class=\"g\">Any brightfield microscope<\/td>\n<td class=\"g\">Any brightfield microscope<\/td>\n<td class=\"m\">Inverted microscope<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3>Step 3 \u2014 Imaging<\/h3>\n<p>Image the wound at T=0 immediately after wound creation. <cite>It is important not to overestimate inhibition of migration due to compound toxicity \u2014 proliferation assays should run in parallel.<\/cite><\/p>\n<p>Any brightfield inverted microscope is sufficient for standard wound healing assay imaging. A fluorescence microscope is only required for fluorescently labelled cells. For automated continuous imaging, in-incubator imagers such as the zenCELL owl image all 24 wells simultaneously without removing the plate from the incubator.<\/p>\n<h3>Step 4 \u2014 Wound Closure Calculation<\/h3>\n<div class=\"formula-box\">\n<div class=\"formula\">Wound Closure (%) = (Initial wound area \u2212 Current wound area) \/ Initial wound area \u00d7 100<\/div>\n<div class=\"formula-note\">Measure at each timepoint. Express as % wound closure over time to generate a wound closure curve.<\/div>\n<\/div>\n<p>For manual analysis, use the free <strong>Wound Healing Size Tool<\/strong> plugin for ImageJ\/Fiji (Suarez-Arnedo et al., PLoS ONE 2020, 900+ citations). For automated analysis, zenCELL owl software calculates gap area, wound closure rate (\u00b5m\u00b2\/h), and t\u00bd gap closure time automatically at every timepoint.<\/p>\n<h2>What Cells Can Be Used in a Wound Healing Assay?<\/h2>\n<div class=\"answer-box\"><span class=\"al\">Quick Answer<\/span>Any adherent cell line can be used \u2014 provided it forms a confluent monolayer. Suspension cells are not compatible. The most commonly used cell lines are HaCaT (keratinocytes), HUVEC (endothelial), A549 (lung cancer), MDA-MB-231 (breast cancer), U87-MG (glioblastoma), NIH 3T3 (fibroblasts), and primary cells including neurons (with PDL\/laminin coating).<\/p>\n<\/div>\n<table class=\"comp-table\">\n<thead>\n<tr>\n<th>Cell Type<\/th>\n<th>Cell Line<\/th>\n<th>Application<\/th>\n<th>Recommended ECM Coating<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>Keratinocytes<\/td>\n<td>HaCaT<\/td>\n<td>Skin wound healing, re-epithelialisation<\/td>\n<td>Collagen I or none<\/td>\n<\/tr>\n<tr>\n<td>Endothelial cells<\/td>\n<td>HUVEC, EA.hy926<\/td>\n<td>Angiogenesis, vascular repair<\/td>\n<td>Fibronectin, Gelatin<\/td>\n<\/tr>\n<tr>\n<td>Breast cancer<\/td>\n<td>MDA-MB-231, MCF-7<\/td>\n<td>Metastasis, drug screening<\/td>\n<td>Fibronectin, Collagen IV<\/td>\n<\/tr>\n<tr>\n<td>Lung cancer<\/td>\n<td>A549, H1299<\/td>\n<td>Invasion, EMT studies<\/td>\n<td>Fibronectin<\/td>\n<\/tr>\n<tr>\n<td>Glioblastoma<\/td>\n<td>U87-MG, U251<\/td>\n<td>Brain tumour migration<\/td>\n<td>Laminin, Fibronectin<\/td>\n<\/tr>\n<tr>\n<td>Fibroblasts<\/td>\n<td>NIH 3T3, primary<\/td>\n<td>Connective tissue repair<\/td>\n<td>Fibronectin, Collagen I<\/td>\n<\/tr>\n<tr>\n<td>Primary neurons<\/td>\n<td>iPSC-derived, DRG<\/td>\n<td>Neurite regeneration<\/td>\n<td>Poly-D-Lysine + Laminin<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>The Biggest Problem: Reproducibility<\/h2>\n<p>The wound healing assay&#8217;s greatest limitation is reproducibility of the wound creation step. <cite>Reproducibility is highly dependent on the precision of the researcher, since scratch closure can be affected by the pressure applied and the angle of the pipette tip.<\/cite><\/p>\n<p>Manual pipette scratching produces wound width variability of <strong>\u00b130\u201360% CV<\/strong> between wells, operators, and experiments. This means:<\/p>\n<ul>\n<li>Any drug effect smaller than 30% is <strong>statistically invisible<\/strong> \u2014 below assay noise<\/li>\n<li>Inter-experiment comparisons are unreliable \u2014 different operators produce different baseline wounds<\/li>\n<li>Publication requires extensive replication to achieve statistical power<\/li>\n<\/ul>\n<h3>How to Standardize Your Wound Healing Assay<\/h3>\n<p>Three approaches improve reproducibility, in order of effectiveness:<\/p>\n<ol>\n<li><strong>Photochemical wound creation<\/strong> \u2014 light mask defines identical wound geometry in every well. CV below 5%. Independent of operator. Compatible with any brightfield microscope.<\/li>\n<li><strong>Insert-based methods<\/strong> \u2014 silicone inserts create a defined gap. Better than manual scratching but insert removal introduces variability and ECM coating is blocked beneath the insert.<\/li>\n<li><strong>Multichannel pipettes + ruler guides<\/strong> \u2014 reduces but does not eliminate manual variability. Still depends on operator pressure and tip angle.<\/li>\n<\/ol>\n<div class=\"cta-box\">\n<div>\n<h3>Download: Wound Healing Assay Method Comparison<\/h3>\n<p>Free 2-page PDF \u2014 where each method fails and how photochemical wound creation solves each problem.<\/p>\n<\/div>\n<p><a href=\"https:\/\/zencellowl.com\/wp-content\/uploads\/2026\/07\/Migration-Assay-Comparison-and-Solution-2026.pdf\">Download Free PDF \u2192<\/a><\/p>\n<\/div>\n<h2>Do I Need a Fluorescence Microscope for a Wound Healing Assay?<\/h2>\n<div class=\"answer-box\"><span class=\"al\">Quick Answer<\/span>No \u2014 a standard brightfield microscope is sufficient for wound healing assay imaging. A fluorescence microscope is only needed if you are imaging fluorescently labelled cells. For photochemical wound creation, a ~395 nm light source is needed (LED lamp, Hg-vapour lamp DAPI channel, or 405 nm laser) \u2014 a full fluorescence microscope is not required.<\/p>\n<\/div>\n<h2>Wound Healing Assay vs. Transwell Migration Assay<\/h2>\n<p>These two assays are often confused but measure fundamentally different processes:<\/p>\n<table class=\"comp-table\">\n<thead>\n<tr>\n<th>Feature<\/th>\n<th>Wound Healing Assay<\/th>\n<th>Transwell \/ Boyden Chamber<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td class=\"cr\">Migration type<\/td>\n<td>Collective \u2014 sheet of cells<\/td>\n<td>Individual \u2014 single cells<\/td>\n<\/tr>\n<tr>\n<td class=\"cr\">Biological model<\/td>\n<td>Wound re-epithelialisation, collective invasion<\/td>\n<td>Chemotaxis, single-cell invasion<\/td>\n<\/tr>\n<tr>\n<td class=\"cr\">Kinetic data<\/td>\n<td>Yes \u2014 full time course<\/td>\n<td>No \u2014 endpoint only<\/td>\n<\/tr>\n<tr>\n<td class=\"cr\">Equipment<\/td>\n<td>Brightfield microscope<\/td>\n<td>Staining + microscope or plate reader<\/td>\n<\/tr>\n<tr>\n<td class=\"cr\">Throughput<\/td>\n<td>Up to 96 wells (photochemical)<\/td>\n<td>Limited \u2014 manual staining and counting<\/td>\n<\/tr>\n<tr>\n<td class=\"cr\">Best for<\/td>\n<td>Drug effects on migration, wound healing, re-epithelialisation<\/td>\n<td>Chemotaxis index, invasion through matrix<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>How to Automate Your Wound Healing Assay<\/h2>\n<p>Manual wound healing assays require removing the plate from the incubator at each timepoint \u2014 causing temperature drop, CO\u2082 loss, and humidity changes that affect cell behaviour. The critical early migration events in the first 2\u20136 hours are routinely missed.<\/p>\n<p>In-incubator live cell imagers solve this by imaging cells continuously inside the incubator. The zenCELL owl images all 24 wells simultaneously at intervals as short as 1 minute, generating complete wound closure curves without any manual intervention. Gap area, migration rate, and t\u00bd closure time are calculated automatically.<\/p>\n<div class=\"cta-box\">\n<div>\n<h3>See automated wound healing assay imaging live<\/h3>\n<p>Free 30-min remote demo via MS Teams \u2014 real cells, real data, your questions answered.<\/p>\n<\/div>\n<p><a href=\"https:\/\/zencellowl.com\/live-remotedemo\/\">Book Free Demo \u2192<\/a><\/p>\n<\/div>\n<h2>Frequently Asked Questions<\/h2>\n<h3>How long does a wound healing assay take?<\/h3>\n<p>Typical duration is 12\u201348 hours depending on cell type and migration speed. Fast-migrating cell lines such as MDA-MB-231 may close wounds in 12\u201324h. Slower cell types such as primary keratinocytes may require 48\u201372h. The assay ends when the wound is fully closed or at a defined timepoint.<\/p>\n<h3>How do I stop cells from proliferating during the assay?<\/h3>\n<p>To ensure wound closure is driven by migration rather than proliferation, treat cells with a proliferation inhibitor such as mitomycin C (10 \u00b5g\/mL for 2h) before wound creation. This is especially important for assays exceeding 24 hours. Always run parallel proliferation controls.<\/p>\n<h3>What concentration of serum should I use during the assay?<\/h3>\n<p>Use reduced serum (0.5\u20131% FBS) or serum-free medium during the migration phase if you want to measure chemotaxis toward a serum gradient. For standard wound closure assays measuring collective migration, normal growth medium (10% FBS) is appropriate.<\/p>\n<h3>Can I perform a wound healing assay in a 96-well plate?<\/h3>\n<p>Yes \u2014 with photochemical wound creation (ScratchMaker plates), 96-well wound healing assays are practical and fully scalable. Manual pipette scratching in 96-well format is impractical due to the number of wells and increased operator variability. Insert-based methods require manual insert handling per well.<\/p>\n<h3>What is a good positive control for a wound healing assay?<\/h3>\n<p>Common positive controls include TGF-\u03b2 (stimulates migration), EGF (epidermal growth factor), and scratch-wound conditioned medium. For inhibition assays, cytochalasin D (actin polymerisation inhibitor) is a standard negative control that blocks migration completely.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Cell Migration \u00b7 Wound Healing \u00b7 Scratch Assay Wound Healing Assay \u2014 Complete Guide: Protocol, Methods &amp; Reproducibility The wound healing assay \u2014 also called the scratch assay or gap [&hellip;]<\/p>\n","protected":false},"author":7,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[10],"tags":[],"class_list":["post-7076","post","type-post","status-publish","format-standard","hentry","category-nicht-kategorisiert-en"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Wound Healing Assay \u2014 Complete Protocol &amp; Method Guide 2026 - zenCELL owl<\/title>\n<meta name=\"description\" content=\"Complete guide to wound healing assays: protocol, methods, reproducibility, wound closure calculation and ImageJ analysis. 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