Record every movement. Calculate speed accurately.
The wound healing, migration assay or scratch assay is a standard method to analyze cell migration in vitro. The dynamics of cell migration into a cell-free area, which has been created by direct manipulation or physical exclusion, are monitored and quantified to analyze cell migration characteristics and to calculate the gap closure time.
Data of Cell Coverage can be used to calculate the “Relative Gap Area” manually. Plotted as a function of time, the time-point of half gap closure (t1/2 Gap Closure) can be determined from this graph.
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