zenCELLOWL · Workflow

Cell Culture Monitoring —

Before & After

See how zenCELLOWL transforms your cell therapy manufacturing process — from manual spot-checks to continuous, documented oversight.

⚠ Current Standard Process

Manual monitoring — blind spots, contamination risk, incomplete documentation
🧫

Cell Seeding

Day 0

Cells are seeded into flasks or bioreactor after isolation. Initial cell count and viability measured manually at seeding.

⚠ Risk

Baseline only. No documentation of early morphological changes in the first 24–48 h.

👁

Manual Microscopy Checks

Intermittent
Scientist removes flask from incubator and checks cells under an external microscope.
⚠ Problems
Incubator opened → temperature & CO₂ disruption. Contamination risk per opening. No data between checks — changes can go undetected for hours.
📋

Manual Cell Count

Intermittent

Trypan blue exclusion or hemocytometer. Performed at irregular intervals, highly operator-dependent.

⚠ Problems
High variability between operators. No continuous growth curve. Missed optimal passage / harvest time points.
🦠

Contamination Detection

Reactive

Contamination is detected visually — often after it has already spread. Media colour change or turbidity is noticed during a routine check.

⚠ Problems

By the time it’s detected, hours or days of culture — and potentially the entire batch — are lost. No early warning system.

🧪

Potency / Cytotoxicity Test

Endpoint Only
CAR-T cells co-cultured with tumor targets. Readout is an endpoint assay (e.g. LDH release) at a fixed time point.
⚠ Problems
Single time point only — killing kinetics and dynamics are invisible. Requires additional assay kits and consumables.
📝

GMP Documentation

Manual

Data entered manually into paper or spreadsheet batch records. Photos taken ad hoc with smartphone.

⚠ Problems

Incomplete, non-standardised records. Time-consuming. Difficult to defend during FDA / EMA audits. Gaps in traceability.
✦ With zenCELLOWL

Continuous, automated, fully documented — inside the incubator, without disruption

🧫

Cell Seeding

Day 0

Cells seeded as normal. zenCELLOWL is already positioned in the incubator — imaging starts automatically at T=0.

✦ Benefit

First image captured within minutes. Morphology and confluence baseline documented automatically — no manual step required.


📡

Continuous In-Incubator Imaging

24 / 7

zenCELLOWL captures images at defined intervals — every 30 min, 1 h, or custom — without ever opening the incubator door.

✦ Benefit

Zero temperature or CO₂ disruption. Zero contamination risk from repeated openings. Up to 48× more data points per day vs. manual checks.

🤖

Automated Cell Count & Growth Curve

AI-Powered
Custom-trained AI model quantifies confluence, counts cells, and tracks proliferation automatically across the full culture period.
✦ Benefit
Continuous, operator-independent growth curves. Optimal passage and harvest time points are flagged automatically — never missed again.

🚨

Early Contamination Alert

Proactive

Morphological deviations, debris accumulation, or abnormal growth patterns are detected within the first imaging interval after onset.

✦ Benefit
Contamination events caught hours earlier. Batch intervention or rescue possible before the culture is lost. Significant cost savings per run.
⚗️

Live Potency / Cytotoxicity Assay

Time-Lapse
CAR-T / tumor co-culture is imaged continuously. Killing kinetics, cell morphology changes, and effector-to-target dynamics are captured in real time.
✦ Benefit
Full killing curve — not just a single endpoint. Richer potency data with fewer additional reagents. Directly supports product release decisions.

Automated GMP Documentation

Audit-Ready

Every image, timestamp, and analysis result is logged automatically. Exportable batch record with standardised morphology reports and time-lapse data.

✦ Benefit
Complete, traceable documentation — zero manual effort. Fully defensible in FDA and EMA audits. Reduces batch release time significantly.

48×

More imaging data points vs. manual checks

0

Incubator openings for monitoring
100%
Automated batch record documentation

Ready to eliminate the blind spots?

See zenCELLOWL in action in your cell therapy application — live demo available.