ScratchMaker scratch assay plates feature photosensitizer-coated bottoms for standardized, reproducible wound healing assays. Available in 6-, 24-, and 96-well format. Requires a fluorescence microscope with blue/violet excitation (~385-408 nm, DAPI channel) – 3 cm distance, 60 sec per well. No washing step or medium change required.
For new customers: we create a personal offer based on your format and quantity needs.
The method you use to create a wound determines what biology you can study. Not all scratch assay plates are equal — the wound type matters. 细胞迁移测定研究 持续表明生理相关性是基于插入和光化学伤口模型之间的关键区别。.
Most labs still create scratch wounds by dragging a pipette tip across the monolayer by hand. This mechanically ruptures cells at the wound edge — width, angle, and depth vary with every pass, every operator, every plate. ScratchMaker scratch assay plates replace this manual step entirely with a precisely defined, reproducible photochemical wound.
"细胞在向伤口移动时，不仅能闭合伤口，还能消化沿途的细胞体。所以我们认为这非常接近生理状况，即生物层面上正在发生的实时情况。"
— 研究人员反馈，zenCELL owl 现场演示 · 2026年6月UV-A/visible light (wavelength typically ~340–408 nm). Standard fluorescence microscopes with Hg-vapour lamp (DAPI filter cube) or 405 nm laser are fully compatible. Photosensitizer absorption maximum: ~395 nm. No dedicated UV lamp or laser required — exposure time 20–60 seconds per well.
约有12,000条PubMed条目涉及划痕实验——其中大多数是使用移液器吸头手动进行的。 实验间变异性是伤口愈合实验出版物中引用最多的局限性。.
相比之下，手动移液器的划痕因操作者的压力、角度和吸头大小不同而存在显著差异——这使得定量分析变得不可靠。.
结果无法在实验、时间点或操作员之间直接比较——破坏了统计能力。.
缺乏标准化是迁移和伤口愈合研究同行评审中经常被拒绝的原因。.
每块板 96 次手动划痕耗时且易出错——高通量筛选不切实际。.
移液器吸头会物理性地撕裂伤口边缘的细胞，产生不受控制的碎片和应激反应，从而混淆迁移读数。.
每口井的划痕时间略有不同，划痕手法也略有不同——时间上的计时和手法上的技巧会随着一个样本板的实验累积而发生漂移。.
Most labs start with the scratch assay plates alone and grow into the full ScratchMaker workflow step by step.
您正在生成可重复的光化学划痕测定伤口——在荧光显微镜下手动对每个孔板成像。.
您的 ScratchMaker 板放置在 zenCELL 猫头鹰内部，它可在 24-72 小时内自动捕捉每个孔的图像，无需手动成像。.
标准定量不足？我们可以为您的细胞类型、伤口几何形状或检测结果构建定制的分析算法——请告知我们您的项目。.
Available in 6-, 24-, and 96-well format. Order plates and masks separately or as a complete Starter Kit. Individual sample offers available for new customers.
Photosensitizer-coated glass bottom plates for standardized, reproducible wound healing assays. Available in 6-, 24-, and 96-well format. No washing step or medium change required after light exposure.
The light mask clips under the ScratchMaker plate and defines exactly where the photosensitizer is activated. Ensures identical wound geometry across all wells, all experiments, all operators. One mask per format — reusable.
Everything you need to run your first standardized scratch assay. Includes ScratchMaker plates in your chosen format, the matching light mask, and a step-by-step protocol. Up to 50% off for new customers.
Ideal for larger cell populations, higher cell input, or applications requiring more image area per well. Compatible with most brightfield and fluorescence microscopes.
The standard format for scratch assay experiments. Fits directly into the zenCELL owl 24-channel imager. Best balance between throughput and image quality.
For high-throughput screening applications. Compatible with zenCELL owl XYZ SCAN stage add-on. Screen up to 96 conditions in a single experiment.
All ScratchMaker plates and masks are available for individual sample offers. Contact us at info@innome.de with your format, quantity, and institution — we will create a personal quote within 24 hours. New customers receive up to 50% off their first Starter Kit order.
ScratchMaker plates with integrated Microelectrode Array (MEA) compatibility — precisely defined wound position relative to the electrode for electrical cell migration measurements. Contact us for early access.
From confluent monolayer to defined photochemical wound – a standardized workflow in four steps. Compatible with 6-, 24-, and 96-well formats.
Seed cells, incubate to ≥95% confluency
Mask clips under the plate, defines exposure zone
Blue/violet light (~408 nm, DAPI channel), 3 cm distance, per well
No washing, no medium change – ready to go
将细胞以适当密度接种到ScratchMaker划痕实验板上。培养直至细胞汇合度达到≥95%——通常需16–24小时，具体时间取决于细胞系。.
将 ScratchMaker 浅膜夹在微孔板底部。该浅膜充当支架和光导管——它确切地定义了每个微孔的哪个区域将被暴露。.
Place each well under your fluorescence microscope light source for 60 seconds at a distance of 3 cm. The blue/violet excitation light resonantly activates the photosensitizer embedded in the glass coating, generating singlet oxygen (¹O₂) precisely within the illuminated area. This induces localized cell death only where the light hits – cells just outside the exposed zone remain completely unaffected.
The scratch assay plate is ready immediately after light exposure. No washing or medium change is required – cells remain in their original medium. Place directly under your microscope or into the zenCELL owl and start live-cell monitoring.
对三种最常见的伤口愈合测定方法的直接比较——以及为什么光化学划痕测定板能产生最符合生理学真实性的数据。.
| 功能 | 刮痕制造板 | 移液器吸头 | Ibidi 插入件 |
|---|---|---|---|
| 伤口类型 | 光化学细胞死亡 | 机械断裂 | 间隙 – 无细胞死亡 |
| 生理学相关性 | 高——模拟体内 | 中等 | 低 - 无碎屑清理 |
| 细胞碎屑清除 | 是的，已定义区域 | 是的，失控 | 不 |
| 插入脱离风险 | 不插入 | 不插入 | 是的——2个多小时后 |
| Scratch 可复现性 | 高 | 低 (±30–60%) | 高 |
| 6孔板 | 是的 | 是的 | 是的 |
| 24孔 | 是的 | 是的 | 是的 |
| 96孔 | 是的 | 费力的 | 是的 |
| 显微镜要求 | 荧光显微镜（最低）· 3厘米距离 | 任何 | 任何 |
| 局部服药 | 是的——逐步暴露井（无菌） | 是的 | 不 |
| 伤口清洗 | 不必 | 必需 | 必需 |
| 每盘价格 | 从 59 欧元起 | ~€0 | 50–80 欧元 |
| 微电极阵列 (MEA) | 兼容 | 替代方法 | 替代方法 |
| 出版适宜性 | 是的 | 有限 | 是的 |
ScratchMaker 板兼容微电极阵列 (MEA) 技术，为基于插入式或手动划痕的电迁移测量方法提供了一种精确的替代方案。由于伤口位置由光掩模精确定义，MEA 电极相对于伤口始终位于同一位置——使得电迁移测量具有更高的可重复性和有效性。.
ScratchMaker 划痕测定板专为直接搭配 zenCELL owl 培养箱成像仪而设计。将板放入其中，按下开始——系统会自动捕获每个孔，每小时一次。.
全自动光化学伤口生成，用于我们的划痕实验板——96个相同的伤口，不到60秒即可完成。目前处于原型开发阶段。.
Illuminate all wells simultaneously in one step using UV-A/visible light (~385–408 nm) illumination. Compatible with all ScratchMaker scratch assay plates – 24-well and 96-well. Early access on request.
关于光源、伤口几何、板块使用和兼容性最常见问题的解答。.
A washing step is not strictly required — cells remain in their original medium and the plate is ready for imaging immediately after light exposure.
However, many researchers perform a medium change before and/or after illumination to remove floating dead cells and improve image quality, as well as to wash out any potentially generated by-products.
When adding reagents such as migration inhibitors or stimulants, a medium change is recommended to introduce them at a defined time point relative to wound creation — either before or after illumination depending on your experimental design.
Protocol guidance validated in collaboration with Stefanie Michaelis, Fraunhofer EMFT / University of Regensburg.
ScratchMaker划痕实验试剂盒价格表、协议PDF及样品订单——请直接联系我们。.
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